Overview

  • Product nameAnti-TIGAR antibody
    See all TIGAR primary antibodies
  • Description
    Rabbit polyclonal to TIGAR
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human TIGAR.

    (Peptide available as ab37909.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab37910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB 1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

  • FunctionProbable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
  • Sequence similaritiesBelongs to the phosphoglycerate mutase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6-bisphosphatase TIGAR antibody
    • C12ORF5 antibody
    • chromosome 12 open reading frame 5 antibody
    • FR2BP antibody
    • Fructose-2,6-bisphosphatase TIGAR antibody
    • Fructose-2,6-bisphosphate 2-phosphatase antibody
    • Probable fructose 2,6 bisphosphatase TIGAR antibody
    • Probable fructose-2 antibody
    • tigar antibody
    • TIGAR_HUMAN antibody
    • TP53 induced glycolysis and apoptosis regulator antibody
    • TP53 induced glycolysis regulatory phosphatase antibody
    • TP53-induced glycolysis and apoptosis regulator antibody
    • Transactivated by NS3TP2 protein antibody
    see all

Anti-TIGAR antibody images

  • ICC/IF image of ab37910 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37910 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-TIGAR antibody (ab37910) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : HEK293 whole cell lysate (ab7902)
    Lane 5 : HepG2 whole cell lysate (ab7900)
    Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ab37910 staining human adrenal tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 1 hour at 25°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C.  A biotinylated goat anti-rabbit antibody diluted 1/400 was used as the secondary.

    See Abreview

References for Anti-TIGAR antibody (ab37910)

This product has been referenced in:
  • Yu HP  et al. TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway. Sci Rep 5:9853 (2015). WB . Read more (PubMed: 25928429) »
  • Zhao Y  et al. Chronic cerebral hypoperfusion causes decrease of O-GlcNAcylation, hyperphosphorylation of tau and behavioral deficits in mice. Front Aging Neurosci 6:10 (2014). WB ; Mouse . Read more (PubMed: 24575038) »

See all 9 Publications for this product

Product Wall

For TIGAR, we recommend any of the lysates shown in the western blot on the datasheet. We do not, however, have free samples.

For GLS2, ab113509 was validated for western blotting on blots of rat kidney lysate. We have this as ab29480.
...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF7 Breast Cancer Cells)
Specification MCF7 Breast Cancer Cells
Fixative Paraformaldehyde
Permeabilization Yes - Methanol - 10 mins
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. Sharon Sneddon

Verified customer

Submitted Nov 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (various incl: HeLa, HEK293, 786-O, MCF-7)
Loading amount 30 µg
Specification various incl: HeLa, HEK293, 786-O, MCF-7
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Oct 07 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenal tumors)
Specification Adrenal tumors
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"