Overview

  • Product nameAnti-TIGAR antibody
    See all TIGAR primary antibodies
  • Description
    Rabbit polyclonal to TIGAR
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) of 19 amino acids from a region near the centre of TIGAR

  • Positive control
    • MCF7 cell lysate Human brain tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

  • FunctionProbable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
  • Sequence similaritiesBelongs to the phosphoglycerate mutase family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6-bisphosphatase TIGAR antibody
    • C12ORF5 antibody
    • chromosome 12 open reading frame 5 antibody
    • FR2BP antibody
    • Fructose-2,6-bisphosphatase TIGAR antibody
    • Fructose-2,6-bisphosphate 2-phosphatase antibody
    • Probable fructose 2,6 bisphosphatase TIGAR antibody
    • Probable fructose-2 antibody
    • tigar antibody
    • TIGAR_HUMAN antibody
    • TP53 induced glycolysis and apoptosis regulator antibody
    • TP53 induced glycolysis regulatory phosphatase antibody
    • TP53-induced glycolysis and apoptosis regulator antibody
    • Transactivated by NS3TP2 protein antibody
    see all

Anti-TIGAR antibody images

  • Lane 1 : Anti-TIGAR antibody (ab62533) at 0.5 µg/ml
    Lane 2 : Anti-TIGAR antibody (ab62533) at 1 µg/ml
    Lane 3 : Anti-TIGAR antibody (ab62533) at 2 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • immunohistochemical analysis of TIGAR expression in human brain tissue using 2.5 µg/ml ab62533.
  • Immunofluorescence of TIGAR in Human Brain cells using ab62533 at 20 ug/ml.

References for Anti-TIGAR antibody (ab62533)

This product has been referenced in:
  • Maiti AK  et al. IL-4 Protects the Mitochondria Against TNFa and IFN? Induced Insult During Clearance of Infection with Citrobacter rodentium and Escherichia coli. Sci Rep 5:15434 (2015). IHC ; Mouse . Read more (PubMed: 26481427) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

Unfortunately, we cannot pinpoint exactly which of our antibodies was used for this publication.

With help of our records we can assume that it was ab62533

http://www.abcam.com/index.html?datash...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"