• Product nameAnti-TIM 1 antibody
    See all TIM 1 primary antibodies
  • Description
    Rabbit polyclonal to TIM 1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid peptide from near the amino terminus of human TIM 1.

  • Positive control
    • Human Uterus Tissue Lysate.



Our Abpromise guarantee covers the use of ab47635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 39 kDa.
IHC-P Use a concentration of 10 µg/ml.


  • FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD365 antibody
    • HAVCR 1 antibody
    • HAVCR antibody
    • HAVcr-1 antibody
    • Havcr1 antibody
    • Hepatitis A virus cellular receptor 1 antibody
    • Kidney injury molecule 1 antibody
    • KIM 1 antibody
    • KIM-1 antibody
    • T cell immunoglobin domain and mucin domain protein 1 antibody
    • T cell immunoglobulin mucin family member 1 antibody
    • T cell immunoglobulin mucin receptor 1 antibody
    • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
    • T-cell membrane protein 1 antibody
    • TIM antibody
    • TIM-1 antibody
    • TIM1 antibody
    • TIMD 1 antibody
    • TIMD-1 antibody
    • TIMD1 antibody
    • TIMD1_HUMAN antibody
    see all

Anti-TIM 1 antibody images

  • Lane 1 : Anti-TIM 1 antibody (ab47635) at 1 µg/ml
    Lane 2 : Anti-TIM 1 antibody (ab47635) at 2 µg/ml

    Lane 1 : Human Uterus Tissue Lysate
    Lane 2 : Human Uterus Tissue Lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 39 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab47635 at 10 ug/ml staining TIM1 in human uterus tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • ICC/IF image of ab47635 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47635, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of TIM-1 in Human Uterus cells using ab47635 at 20 ug/ml.

References for Anti-TIM 1 antibody (ab47635)

ab47635 has not yet been referenced specifically in any publications.

Product Wall

Product ab47635 was raised using N-terminal peptide and immunogen corresponds to the ecto domain of protein Tim 1 (http://www.uniprot.org/uniprot/Q96D42). This antibody has been tested with mouse and human samples in WB, ICC/IF and IHC-P.
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Thank you for contacting abcam.

The image on our datasheet for this product ddoes show high non-specific background as well which seemed to be tamed but not eliminated by using a much lower dilution of the product. In that particular case 1u...

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