Customer reviews and FAQs

Overview

  • Product name
    Anti-TIM 1 antibody
    See all TIM 1 primary antibodies
  • Description
    Mouse monoclonal to TIM 1
  • Tested applications
    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment with tag: KVGGEAGPSV TLPCHYSGAV TSMCWNRGSC SLFTCQNGIV WTNGTHVTYR KDTRYKLLGD LSRRDVSLTI ENTAVSDSGV YCCRVEHRGW FNDMKITVSL, corresponding to amino acids 23-123 of Human TIM 1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • TIM 1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent concentration. The detection limit for recombinant tagged TIM 1 is approximately 10ng/ml if used as a capture antibody.
Flow Cyt Use at an assay dependent concentration. See Abreview.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificity
    Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD365 antibody
      • HAVCR 1 antibody
      • HAVCR antibody
      • HAVcr-1 antibody
      • Havcr1 antibody
      • Hepatitis A virus cellular receptor 1 antibody
      • Kidney injury molecule 1 antibody
      • KIM 1 antibody
      • KIM-1 antibody
      • T cell immunoglobin domain and mucin domain protein 1 antibody
      • T cell immunoglobulin mucin family member 1 antibody
      • T cell immunoglobulin mucin receptor 1 antibody
      • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
      • T-cell membrane protein 1 antibody
      • TIM antibody
      • TIM-1 antibody
      • TIM1 antibody
      • TIMD 1 antibody
      • TIMD-1 antibody
      • TIMD1 antibody
      • TIMD1_HUMAN antibody
      see all

    Anti-TIM 1 antibody images

    • Western blot - TIM 1 antibody (ab66062)
      Western blot - TIM 1 antibody (ab66062)
      All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml

      Lane 1 : TIM 1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      Goat Anti-Mouse IgG at 1/2500 dilution

      Predicted band size : 39 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    References for Anti-TIM 1 antibody (ab66062)

    This product has been referenced in:
    • Xiao X  et al. Aldosterone induces NRK-52E cell apoptosis in acute kidney injury via rno-miR-203 hypermethylation and Kim-1 upregulation. Exp Ther Med 12:915-924 (2016). WB ; Mouse . Read more (PubMed: 27446296) »
    • Liu J  et al. IL-10-producing B cells are induced early in HIV-1 infection and suppress HIV-1-specific T cell responses. PLoS One 9:e89236 (2014). Flow Cyt . Read more (PubMed: 24586620) »

    See all 2 Publications for this product

    Publishing research using ab66062? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

    Submit an abreview for ab66062 Submit a question for ab66062

    2 Abreviews or Q&A

    10
    Votes: Highest First

    My name is Johnny Huang and I’m from the University of Queensland, Australia. I purchased your antibodies against TIM-1 (ab66062 and 47634) recently. I tried the antibodies for western blotting but I couldn’t get single band. In fact, TIM-1 is a gl...

    Read More

    Thank you for contacting us. We find that specific protocol is not necessary for this product. So I have attached the protocol that we generally recommend to our customers. We have used reduced denaturing conditions in WB without deglyco...

    Read More

    Flow Cytometry abreview for Anti-TIM 1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (Peripheral blood mononuclear cells)
    Specification
    Peripheral blood mononuclear cells
    Preparation
    Cell harvesting/tissue preparation method: Ficoll gradient method
    Sample buffer: PBS
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    Lymphocytes and monocytes
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Aug 07 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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