Overview

  • Product nameAnti-TIM 1 antibody
    See all TIM 1 primary antibodies
  • Description
    Mouse monoclonal to TIM 1
  • Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment with tag: KVGGEAGPSV TLPCHYSGAV TSMCWNRGSC SLFTCQNGIV WTNGTHVTYR KDTRYKLLGD LSRRDVSLTI ENTAVSDSGV YCCRVEHRGW FNDMKITVSL, corresponding to amino acids 23-123 of Human TIM 1

  • Positive control
    • TIM 1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent concentration. The detection limit for recombinant tagged TIM 1 is approximately 10ng/ml if used as a capture antibody.
Flow Cyt Use at an assay dependent concentration. See Abreview.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD365 antibody
    • HAVCR 1 antibody
    • HAVCR antibody
    • HAVcr-1 antibody
    • Havcr1 antibody
    • Hepatitis A virus cellular receptor 1 antibody
    • Kidney injury molecule 1 antibody
    • KIM 1 antibody
    • KIM-1 antibody
    • T cell immunoglobin domain and mucin domain protein 1 antibody
    • T cell immunoglobulin mucin family member 1 antibody
    • T cell immunoglobulin mucin receptor 1 antibody
    • T-cell immunoglobulin and mucin domain-containing protein 1 antibody
    • T-cell membrane protein 1 antibody
    • TIM antibody
    • TIM-1 antibody
    • TIM1 antibody
    • TIMD 1 antibody
    • TIMD-1 antibody
    • TIMD1 antibody
    • TIMD1_HUMAN antibody
    see all

Anti-TIM 1 antibody images

  • All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml

    Lane 1 : TIM 1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 39 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-TIM 1 antibody (ab66062)

This product has been referenced in:
  • Liu J  et al. IL-10-producing B cells are induced early in HIV-1 infection and suppress HIV-1-specific T cell responses. PLoS One 9:e89236 (2014). Flow Cyt . Read more (PubMed: 24586620) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. We find that specific protocol is not necessary for this product. So I have attached the protocol that we generally recommend to our customers. We have used reduced denaturing conditions in WB without deglyco...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Peripheral blood mononuclear cells)
Specification Peripheral blood mononuclear cells
Preparation Cell harvesting/tissue preparation method: Ficoll gradient method
Sample buffer: PBS
Fixation none
Permeabilization No
Gating Strategy Lymphocytes and monocytes
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Verified customer

Submitted Aug 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"