- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-TIM 1 antibody
See all TIM 1 primary antibodies -
DescriptionMouse monoclonal to TIM 1
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Host speciesMouse
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Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
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Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment with tag: KVGGEAGPSV TLPCHYSGAV TSMCWNRGSC SLFTCQNGIV WTNGTHVTYR KDTRYKLLGD LSRRDVSLTI ENTAVSDSGV YCCRVEHRGW FNDMKITVSL, corresponding to amino acids 23-123 of Human TIM 1
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Positive control
- TIM 1 transfected 293T cell lysate.
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab190696 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab66062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa). | |
| ELISA | Use at an assay dependent concentration. The detection limit for recombinant tagged TIM 1 is approximately 10ng/ml if used as a capture antibody. | |
| Flow Cyt | Use at an assay dependent concentration. See Abreview.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
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Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
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Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 26762 Human
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- Unigene: 129711 Human
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Alternative names
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
see all
Images
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Western blot - TIM 1 antibody (ab66062)All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml
Lane 1 : TIM 1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 39 kDa
Observed band size: 36 kDa (why is the actual band size different from the predicted?)
References
This product has been referenced in:
- Xiao X et al. Aldosterone induces NRK-52E cell apoptosis in acute kidney injury via rno-miR-203 hypermethylation and Kim-1 upregulation. Exp Ther Med 12:915-924 (2016). WB ; Mouse . Read more (PubMed: 27446296) »
- Liu J et al. IL-10-producing B cells are induced early in HIV-1 infection and suppress HIV-1-specific T cell responses. PLoS One 9:e89236 (2014). Flow Cyt . Read more (PubMed: 24586620) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"