- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-TIM 1 antibody
See all TIM 1 primary antibodies - DescriptionMouse monoclonal to TIM 1
- Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
- Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- Immunogen
Recombinant fragment with tag: KVGGEAGPSV TLPCHYSGAV TSMCWNRGSC SLFTCQNGIV WTNGTHVTYR KDTRYKLLGD LSRRDVSLTI ENTAVSDSGV YCCRVEHRGW FNDMKITVSL, corresponding to amino acids 23-123 of Human TIM 1
- Positive control
- TIM 1 transfected 293T cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- ClonalityMonoclonal
- IsotypeIgG2a
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab66062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa). | |
| ELISA | Use at an assay dependent concentration. The detection limit for recombinant tagged TIM 1 is approximately 10ng/ml if used as a capture antibody. | |
| Flow Cyt | Use at an assay dependent concentration. See Abreview. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
- FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
- Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
- Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. - Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 26762 Human
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- Unigene: 129711 Human
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Alternative names
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
see all
Anti-TIM 1 antibody images
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Western blot - TIM 1 antibody (ab66062)All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml
Lane 1 : TIM 1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size : 39 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
References for Anti-TIM 1 antibody (ab66062)
This product has been referenced in:
- Xiao X et al. Aldosterone induces NRK-52E cell apoptosis in acute kidney injury via rno-miR-203 hypermethylation and Kim-1 upregulation. Exp Ther Med 12:915-924 (2016). WB ; Mouse . Read more (PubMed: 27446296) »
- Liu J et al. IL-10-producing B cells are induced early in HIV-1 infection and suppress HIV-1-specific T cell responses. PLoS One 9:e89236 (2014). Flow Cyt . Read more (PubMed: 24586620) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"