The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.
Use a concentration of 2.5 - 5 µg/ml.
FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
T cell immunoglobin domain and mucin domain protein 1 antibody
T cell immunoglobulin mucin family member 1 antibody
T cell immunoglobulin mucin receptor 1 antibody
T-cell immunoglobulin and mucin domain-containing protein 1 antibody
T-cell membrane protein 1 antibody
TIMD 1 antibody
Anti-TIM 1 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 1 antibody (ab78494)Image is courtesy of an anonymous AbReview.
Immunohistochemical analysis of Formaldehyde-fixed murine obese nephric tissue sections, labelling TIM 1 with ab78494 at a dilution of 1/2500 incubated for 12 hours at 4°C in PBS and 2.5% Horse serum. Heat mediated antigen retrival was with Citrate buffer. Blocking was with 2.5% horse serum incubated for 1 hour at 25°C. Secondary was a horse anti-rabbit polyclonal HRP conjugate used undiluted. Counterstaining against nuclear DNA was with DAPI.