Overview

  • Product name
  • Description
    Rabbit polyclonal to TIM 4
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 aminoacid synthetic peptide from near the center of human TIM 4

  • Positive control
    • Jurkat cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain
    Recognizes phosphatidyl serine via its immunoglobulin domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • SMUCKLER antibody
    • Spleen, mucin-containing, knockout of lymphotoxin protein antibody
    • T cell immunoglobulin and mucin domain containing protein 4 antibody
    • T-cell immunoglobulin and mucin domain containing 4 antibody
    • T-cell immunoglobulin and mucin domain containing molecule antibody
    • T-cell immunoglobulin and mucin domain-containing protein 4 antibody
    • T-cell immunoglobulin and mucin domains-containing protein 4 antibody
    • T-cell membrane protein 4 antibody
    • TIM-4 antibody
    • Tim4 antibody
    • TIMD 4 antibody
    • TIMD-4 antibody
    • Timd4 antibody
    • TIMD4_HUMAN antibody
    see all

Anti-TIM 4 antibody images



  • Predicted band size : 42 kDa
    Western Blot of RAW264.7 cell lysate labeling TIM 4 with Anti-TIM 4 antibody (ab47637) at 2µg/ml in (1) the absence and (2) the presence of blocking peptide.
  • Lane 1 : Anti-TIM 4 antibody (ab47637) at 1 µg/ml
    Lane 2 : Anti-TIM 4 antibody (ab47637) at 2 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa,30 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab47637 at 10µg/ml staining TIM 4 in Jurkat cells by ICC/IF

  • Immunofluorescence of TIM-4 in Jurkat cells using ab47637 at 20 ug/ml.

References for Anti-TIM 4 antibody (ab47637)

This product has been referenced in:
  • Mukherjee S  et al. Boosting efferocytosis in alveolar space using BCG vaccine to protect host against influenza pneumonia. PLoS One 12:e0180143 (2017). WB ; Mouse . Read more (PubMed: 28686604) »
  • Park SY  et al. Stabilin-2 modulates the efficiency of myoblast fusion during myogenic differentiation and muscle regeneration. Nat Commun 7:10871 (2016). Read more (PubMed: 26972991) »

See all 4 Publications for this product

Product Wall

Thank you for your inquiry. I heard back from the supplying lab that ab47637 is against a peptide from the extracellular portion of TIM4. In case you need to know, the other one in our catalogue, ab47636, is against the cytoplasmic domain. I hop...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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