Overview

  • Product nameAnti-TIM 4 antibody
    See all TIM 4 primary antibodies
  • Description
    Rabbit polyclonal to TIM 4
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 aminoacid synthetic peptide from near the center of human TIM 4

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab47637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPhosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • DomainRecognizes phosphatidyl serine via its immunoglobulin domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • SMUCKLER antibody
    • Spleen, mucin-containing, knockout of lymphotoxin protein antibody
    • T cell immunoglobulin and mucin domain containing protein 4 antibody
    • T-cell immunoglobulin and mucin domain containing 4 antibody
    • T-cell immunoglobulin and mucin domain containing molecule antibody
    • T-cell immunoglobulin and mucin domain-containing protein 4 antibody
    • T-cell immunoglobulin and mucin domains-containing protein 4 antibody
    • T-cell membrane protein 4 antibody
    • TIM-4 antibody
    • Tim4 antibody
    • TIMD 4 antibody
    • TIMD-4 antibody
    • Timd4 antibody
    • TIMD4_HUMAN antibody
    see all

Anti-TIM 4 antibody images

  • Lane 1 : Anti-TIM 4 antibody (ab47637) at 1 µg/ml
    Lane 2 : Anti-TIM 4 antibody (ab47637) at 2 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa,30 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab47637 at 10µg/ml staining TIM 4 in Jurkat cells by ICC/IF

  • Immunofluorescence of TIM-4 in Jurkat cells using ab47637 at 20 ug/ml.

References for Anti-TIM 4 antibody (ab47637)

This product has been referenced in:
  • Kim HS  et al. T cell Ig domain and mucin domain 1 engagement on invariant NKT cells in the presence of TCR stimulation enhances IL-4 production but inhibits IFN-gamma production. J Immunol 184:4095-106 (2010). Flow Cyt ; Mouse . Read more (PubMed: 20220086) »
  • Xu L  et al. T cell immunoglobulin- and mucin-domain-containing molecule-4 attenuates concanavalin A-induced hepatitis by regulating macrophage. J Leukoc Biol 88:329-36 (2010). WB ; Mouse . Read more (PubMed: 20453106) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry. I heard back from the supplying lab that ab47637 is against a peptide from the extracellular portion of TIM4. In case you need to know, the other one in our catalogue, ab47636, is against the cytoplasmic domain. I hop...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"