Anti-Timeless antibody [EPR5275] (ab109512)


  • Product nameAnti-Timeless antibody [EPR5275]
    See all Timeless primary antibodies
  • Description
    Rabbit monoclonal [EPR5275] to Timeless
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human Timeless.

  • Positive control
    • HeLa, Jurkat, JAR, and BxPC-3 cell lysates, Human colon and Human tonsil tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionRequired for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
    • Tissue specificityExpressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis. Highest levels of expression in placenta, pancreas, thymus and testis.
    • Sequence similaritiesBelongs to the timeless family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ12640 antibody
      • FLJ20714 antibody
      • hTIM antibody
      • Protein timeless homolog antibody
      • TIM antibody
      • TIM_HUMAN antibody
      • TIM1 antibody
      • Timeless antibody
      • timeless circadian clock 1 antibody
      • timeless circadian clock antibody
      • timeless homolog antibody
      • TIMELESS1 antibody
      • Tof1 homolog antibody
      see all

    Anti-Timeless antibody [EPR5275] images

    • All lanes : Anti-Timeless antibody [EPR5275] (ab109512) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 139 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109512 at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109512 at a dilution of 1/100.

    References for Anti-Timeless antibody [EPR5275] (ab109512)

    ab109512 has not yet been referenced specifically in any publications.

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