Overview

  • Product nameAnti-TIMM10 antibody
    See all TIMM10 primary antibodies
  • Description
    Rabbit polyclonal to TIMM10
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: MDPLRAQQLA AELEVEMMAD MYNRMTSACH RKCVPPHYKE AELSKGESVC LDRCVSKYLD IHERMGKKLT ELSMQDEELM KRVQQSSGPA, corresponding to N terminal amino acids 1-90 of Human TIMM10.

  • Positive control
    • Human Kidney tissue; RT 4, U 251 MG, Human Liver and Human Tonsil lysates; U 2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab122606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

  • FunctionMitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.
  • Tissue specificityUbiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the small Tim family.
  • DomainThe twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM10 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mitochondrial import inner membrane translocase subunit tim10 antibody
    • TIM10_HUMAN antibody
    • TIM10A antibody
    • TIMM10 antibody
    • Translocase of inner mitochondrial membrane 10 homolog (yeast) [ antibody
    see all

Anti-TIMM10 antibody images

  • ab122606, at 1/50 dilution, staining TIMM10 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab122606, at 4µg/ml, staining TIMM10 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
  • All lanes : Anti-TIMM10 antibody (ab122606) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

References for Anti-TIMM10 antibody (ab122606)

ab122606 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"