Overview

  • Product name
  • Description
    Rabbit polyclonal to TIMM13
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 5-54 (FGSDFGGTGG GKLDPGAIME QVKVQIAVAN AQELLQRMTD KCFRKCIGKP)of Mouse TIMM13 (NP_038923).

  • Positive control
    • Mouse Kidney Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins.
  • Tissue specificity
    Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
  • Sequence similarities
    Belongs to the small Tim family.
  • Domain
    The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Mitochondrial import inner membrane translocase subunit TIM13 antibody
    • Mitochondrial import inner membrane translocase subunit Tim13B antibody
    • ppv1 antibody
    • TIM 13 antibody
    • TIM13 antibody
    • TIM13_HUMAN antibody
    • TIM13B antibody
    • TIMM 13 antibody
    • Timm13 antibody
    • TIMM13A antibody
    • TIMM13B antibody
    • Translocase of inner mitochondrial membrane 13 homolog antibody
    see all

Images

  • Anti-TIMM13 antibody (ab113781) at 1 µg/ml + Mouse Kidney Lysate at 10 µg

    Predicted band size : 11 kDa

References

ab113781 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart (Embryonic))
Permeabilization
Yes - Tween-20
Specification
Heart (Embryonic)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 29 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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