Overview

  • Product name
  • Description
    Mouse polyclonal to TIMM13
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) with tag

  • Positive control
    • Recombinant tagged TIMM13.

Properties

Applications

Our Abpromise guarantee covers the use of ab66142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: 1/1000 - 1/2500. Predicted molecular weight: 10 kDa.

    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins.
    • Tissue specificity
      Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.
    • Sequence similarities
      Belongs to the small Tim family.
    • Domain
      The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Mitochondrial import inner membrane translocase subunit TIM13 antibody
      • Mitochondrial import inner membrane translocase subunit Tim13B antibody
      • ppv1 antibody
      • TIM 13 antibody
      • TIM13 antibody
      • TIM13_HUMAN antibody
      • TIM13B antibody
      • TIMM 13 antibody
      • Timm13 antibody
      • TIMM13A antibody
      • TIMM13B antibody
      • Translocase of inner mitochondrial membrane 13 homolog antibody
      see all

    Anti-TIMM13 antibody images



    • Predicted band size : 10 kDa

    References for Anti-TIMM13 antibody (ab66142)

    ab66142 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - other (HEK293)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Other - Protein G dynabeads
    Specification
    HEK293
    Username

    Dr. Edyta Marcon

    Verified customer

    Submitted Jun 26 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up