Recombinant
RabMAb

Anti-TIMM17A antibody [EPR16098] (ab192246)

Overview

  • Product name
    Anti-TIMM17A antibody [EPR16098]
    See all TIMM17A primary antibodies
  • Description
    Rabbit monoclonal [EPR16098] to TIMM17A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TIMM17A aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99595

  • Positive control
    • HeLa, K562, Raw264.7, PC-12 and NIH/3T3 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
IP 1/40.
WB 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).

Target

Images

  • All lanes : Anti-TIMM17A antibody [EPR16098] (ab192246) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 17 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-TIMM17A antibody [EPR16098] (ab192246) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 17 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling TIMM17A with ab192246 at 1/50 dilution (11.7 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

    The two negative controls are ab192246 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

  • Western blot analysis of TIMM17A in HeLa cell lysates immunoprecipitated using ab192246 at 1/40 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate.

     

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Blocking/Dilution buffer: 5% NFDM /TBST.

References

ab192246 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Monkey Tissue lysate - whole (Heart (Rhesus macaque))
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Heart (Rhesus macaque)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Dec 29 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Dec 28 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Dec 28 2017

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Oct 24 2017

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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