Recombinant
Anti-TIMM22 antibody [EPR9973] (ab167423)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-TIMM22 antibody [EPR9973]
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DescriptionRabbit monoclonal [EPR9973] to TIMM22
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to residues in Human TIMM22 (UniProt: Q9Y584)
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Positive control
- A673, T47D, A431 and BxPC-3 cell lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR9973
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab167423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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RelevanceTIMM22 is an essential core component of the TIM22 protein insertion complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated, signal-gated hydrophilic high-conductance channel. It forms a twin-pore translocase that uses the membrane potential as an external driving force in 2 voltage-dependent steps.
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Cellular localizationMitochondrion inner membrane; Multi-pass membrane protein.
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Database links
- Entrez Gene: 29928 Human
- Entrez Gene: 56322 Mouse
- Entrez Gene: 79463 Rat
- Omim: 607251 Human
- SwissProt: Q9Y584 Human
- SwissProt: Q9CQ85 Mouse
- SwissProt: Q9JKW1 Rat
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Alternative names
- Mitochondrial import inner membrane translocase subunit Tim22 antibody
- Putative membrane protein antibody
- Testis expressed sequence 4 antibody
see all
Images
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![Western blot - Anti-TIMM22 antibody [EPR9973] (ab167423)](http://a.abcam.com/ps/datasheet/images/167/ab167423/TIMM22-Primary-antibodies-ab167423-1.jpg)
Western blot - Anti-TIMM22 antibody [EPR9973] (ab167423)All lanes : Anti-TIMM22 antibody [EPR9973] (ab167423) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : T47D cell lysate
Lane 3 : A431 cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 20 kDa
Protocols
References
This product has been referenced in:
- Thompson K et al. Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number. Am J Hum Genet 99:860-876 (2016). Read more (PubMed: 27693233) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"