Overview

  • Product nameAnti-TIMM23 antibody
  • Description
    Rabbit polyclonal to TIMM23
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TIMM23 aa 130-158 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O14925

  • Positive control
    • WB: MCF7 and A431 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab116329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 22 kDa.

Target

  • RelevanceTIMM23 belongs to the Tim17/Tim22/Tim23 family and is an essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
  • Cellular localizationMitochondrion inner membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • MGC22767 antibody
    • MGC93478 antibody
    • Mitochondrial import inner membrane translocase subunit Tim23 antibody
    • Predicted protein of HQ1197 antibody
    • PRO1197 antibody
    • TIM23 antibody
    • TIMM 23 antibody
    • TIMM23 antibody
    • Translocase of inner mitochondrial membrane 23 (yeast) homolog antibody
    • Translocase of inner mitochondrial membrane 23 homolog (yeast) antibody
    • Translocase of inner mitochondrial membrane 23 homolog B antibody
    • Translocation of mitochondrial precursor proteins antibody
    see all

Anti-TIMM23 antibody images

  • All lanes : Anti-TIMM23 antibody (ab116329) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 22 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa


    Exposure time : 3 seconds

    Western blot analysis of NB4 human cell line (cell fractionation, 30µg/lane) labelling TIMM23 with ab116329 at 1/500.  ab97051 was used as the secondary antibody (1/10000).

    Treatment - DMSO Control (CTL), 0,5 mM Hydroxyurea (HU), 1 µM Doxorubicin (DR).

    See Abreview

References for Anti-TIMM23 antibody (ab116329)

This product has been referenced in:
  • Yano H  et al. Inhibition of mitochondrial protein import by mutant huntingtin. Nat Neurosci 17:822-31 (2014). ICC/IF ; Mouse . Read more (PubMed: 24836077) »
  • Zhang J  et al. UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells. EMBO J 30:4860-73 (2011). Read more (PubMed: 22085932) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (NB4 human cell line, cell fractionation)
Loading amount 30 µg
Specification NB4 human cell line, cell fractionation
Treatment DMSO Control (CTL), 0,5 mM Hydroxyurea (HU), 1 µM Doxorubicin (DR)
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

Submitted Feb 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"