Overview

  • Product name
    Anti-TIMM23 antibody
  • Description
    Rabbit polyclonal to TIMM23
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TIMM23 aa 130-158 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O14925

  • Positive control
    • WB: MCF7 and A431 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 22 kDa.

Target

  • Relevance
    TIMM23 belongs to the Tim17/Tim22/Tim23 family and is an essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
  • Cellular localization
    Mitochondrion inner membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • MGC22767 antibody
    • MGC93478 antibody
    • Mitochondrial import inner membrane translocase subunit Tim23 antibody
    • Predicted protein of HQ1197 antibody
    • PRO1197 antibody
    • TIM23 antibody
    • TIMM 23 antibody
    • TIMM23 antibody
    • Translocase of inner mitochondrial membrane 23 (yeast) homolog antibody
    • Translocase of inner mitochondrial membrane 23 homolog (yeast) antibody
    • Translocase of inner mitochondrial membrane 23 homolog B antibody
    • Translocation of mitochondrial precursor proteins antibody
    see all

Images

  • All lanes : Anti-TIMM23 antibody (ab116329) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 22 kDa

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa


    Exposure time : 3 seconds

    Western blot analysis of NB4 human cell line (cell fractionation, 30µg/lane) labelling TIMM23 with ab116329 at 1/500.  ab97051 was used as the secondary antibody (1/10000).

    Treatment - DMSO Control (CTL), 0,5 mM Hydroxyurea (HU), 1 µM Doxorubicin (DR).

    See Abreview

References

This product has been referenced in:
  • Wai T  et al. The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L. EMBO Rep 17:1844-1856 (2016). Read more (PubMed: 27737933) »
  • Yano H  et al. Inhibition of mitochondrial protein import by mutant huntingtin. Nat Neurosci 17:822-31 (2014). ICC/IF ; Mouse . Read more (PubMed: 24836077) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (human fibroblast cell and mitochondria fraction is)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
human fibroblast cell and mitochondria fraction is
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 09 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (NB4 human cell line, cell fractionation)
Loading amount
30 µg
Specification
NB4 human cell line, cell fractionation
Treatment
DMSO Control (CTL), 0,5 mM Hydroxyurea (HU), 1 µM Doxorubicin (DR)
Gel Running Conditions
Reduced Denaturing (13%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

Submitted Feb 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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