Overview

  • Product name
  • Description
    Mouse polyclonal to TIMM44
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to amino acids 1-452 of Human TIMM44 (NP_006342.2).

  • Positive control
    • TIMM44 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab168649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.

Target

  • Relevance
    TIMM44 is the essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. It recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
  • Cellular localization
    Mitochondrion inner membrane.
  • Database links
  • Alternative names
    • DKFZp686H05241 antibody
    • MIMT44 antibody
    • Mitochondrial inner membrane translocase antibody
    • TIM44 antibody
    • Translocase of inner mitochondrial membrane 44 antibody
    • Translocase of inner mitochondrial membrane 44 homolog (yeast) antibody
    see all

Images

  • All lanes : Anti-TIMM44 antibody (ab168649) at 1 µg/ml

    Lane 1 : TIMM44 transfected 293T cell lysate

    Lane 2 : Non-transfected 293T cell lysate


    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP
    at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 51 kDa

References

This product has been referenced in:
  • Vais H  et al. EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. Cell Rep 14:403-410 (2016). Read more (PubMed: 26774479) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Mouse brain homogenate, Mitochondria from mouse br)
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE, 55C for 10 min denaturation)
Loading amount
20 µg
Specification
Mouse brain homogenate, Mitochondria from mouse br
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Svitlana Yablonska

Verified customer

Submitted Jul 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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