Recombinant
RabMAb

Anti-TIMM44 antibody [EPR16821] (ab194829)

Overview

  • Product name
    Anti-TIMM44 antibody [EPR16821]
    See all TIMM44 primary antibodies
  • Description
    Rabbit monoclonal [EPR16821] to TIMM44
  • Tested applications
    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human TIMM44 aa 1-250. The exact sequence is proprietary.
    Database link: O43615

  • Positive control
    • A431, HepG2 and K562 cell lysates; Mouse heart lysate; Human endometrial adenocarcinoma tissue; A431 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16821
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
ICC/IF 1/50.
IP 1/20.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance
    TIMM44 is the essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. It recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
  • Cellular localization
    Mitochondrion inner membrane.
  • Database links
  • Alternative names
    • DKFZp686H05241 antibody
    • MIMT44 antibody
    • Mitochondrial inner membrane translocase antibody
    • TIM44 antibody
    • Translocase of inner mitochondrial membrane 44 antibody
    • Translocase of inner mitochondrial membrane 44 homolog (yeast) antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling TIMM44 with ab194829 at 1/100 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194829. Cytoplasm staining on humanendometrial adenocarcinoma was observed.

  • Anti-TIMM44 antibody [EPR16821] (ab194829) at 1/1000 dilution + mouse heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 51 kDa
  • Immunofluorescent analysis of A431 cells labeling TIMM44 with ab194829 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on A431 cell line was observed.

  • All lanes : Anti-TIMM44 antibody [EPR16821] (ab194829) at 1/5000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 51 kDa
  • Immunoprecipitation analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab194826 at 1/20 dilution (Lane 1).  Lane 2: PBS instead of K562 cell lysate. ab194829 at 1/1000 was used for subsequent western blot detection. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

References

ab194829 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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