Overview

  • Product nameAnti-TIMM50 antibody [EPR5784]
    See all TIMM50 primary antibodies
  • Description
    Rabbit monoclonal [EPR5784] to TIMM50
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human TIMM50.

  • Positive control
    • 293T, SH SY5Y, HepG2 and HeLa cell lysates Human kidney tissue, human liver tissue (IHC-P)
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionEssential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
    • Tissue specificityWidely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
    • Sequence similaritiesBelongs to the TIM50 family.
      Contains 1 FCP1 homology domain.
    • DomainThe FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
    • Cellular localizationMitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
    • Information by UniProt
    • Database links
    • Alternative names
      • Homolog of yeast Tim50 antibody
      • Import inner membrane translocase subunit TIM 50 antibody
      • Import inner membrane translocase subunit TIM50 antibody
      • Import inner membrane translocase subunit TIM50 mitochondrial antibody
      • MGC102733 antibody
      • Mitochondrial import inner membrane translocase subunit TIM50 antibody
      • TIM 50 antibody
      • TIM 50L antibody
      • TIM50 antibody
      • Tim50 like protein antibody
      • TIM50_HUMAN antibody
      • TIM50L antibody
      • TIMM 50 antibody
      • TIMM50 antibody
      • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
      • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
      • Translocase of inner mitochondrial membrane 50 homolog antibody
      • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
      see all

    Anti-TIMM50 antibody [EPR5784] images

    • All lanes : Anti-TIMM50 antibody [EPR5784] (ab109527) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 40 kDa
    • ab109527, at 1/50, staining TIMM50 in paraffin-embedded kidney tissue by Immunohistochemistry.
    • ab109527, at 1/50, staining TIMM50 in paraffin-embedded liver tissue by Immunohistochemistry.

    References for Anti-TIMM50 antibody [EPR5784] (ab109527)

    ab109527 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"