Overview

  • Product nameAnti-TIMM50 antibody [EPR5785]
    See all TIMM50 primary antibodies
  • Description
    Rabbit monoclonal [EPR5785] to TIMM50
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human TIMM50

  • Positive control
    • 293T, SH-SY5Y, HepG2, and HeLa cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionEssential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
    • Tissue specificityWidely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
    • Sequence similaritiesBelongs to the TIM50 family.
      Contains 1 FCP1 homology domain.
    • DomainThe FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
    • Cellular localizationMitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
    • Information by UniProt
    • Database links
    • Alternative names
      • Homolog of yeast Tim50 antibody
      • Import inner membrane translocase subunit TIM 50 antibody
      • Import inner membrane translocase subunit TIM50 antibody
      • Import inner membrane translocase subunit TIM50 mitochondrial antibody
      • MGC102733 antibody
      • Mitochondrial import inner membrane translocase subunit TIM50 antibody
      • TIM 50 antibody
      • TIM 50L antibody
      • TIM50 antibody
      • Tim50 like protein antibody
      • TIM50_HUMAN antibody
      • TIM50L antibody
      • TIMM 50 antibody
      • TIMM50 antibody
      • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
      • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
      • Translocase of inner mitochondrial membrane 50 homolog antibody
      • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
      see all

    Anti-TIMM50 antibody [EPR5785] images

    • All lanes : Anti-TIMM50 antibody [EPR5785] (ab109436) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-TIMM50 antibody [EPR5785] (ab109436)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Thank you very much for your call yesterday.

    I've heard back from the lab, and the concentration of this lot is 0.932 mg/mL. The hybridoma cells are grown in serum-free media, so the supernatant does not contain any media.

    I hope th...

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