Recombinant
RabMAb

Anti-TIMM50 antibody [EPR5785] (ab109436)

Overview

  • Product name
    Anti-TIMM50 antibody [EPR5785]
    See all TIMM50 primary antibodies
  • Description
    Rabbit monoclonal [EPR5785] to TIMM50
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human TIMM50

  • Positive control
    • 293T, SH-SY5Y, HepG2, and HeLa cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
    • Tissue specificity
      Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level).
    • Sequence similarities
      Belongs to the TIM50 family.
      Contains 1 FCP1 homology domain.
    • Domain
      The FCP1 homology domain does not contain the canonical D-x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity in vivo.
    • Cellular localization
      Mitochondrion inner membrane and Nucleus speckle. Nuclear and enriched in speckles with snRNPs.
    • Information by UniProt
    • Database links
    • Alternative names
      • Homolog of yeast Tim50 antibody
      • Import inner membrane translocase subunit TIM 50 antibody
      • Import inner membrane translocase subunit TIM50 antibody
      • Import inner membrane translocase subunit TIM50 mitochondrial antibody
      • MGC102733 antibody
      • Mitochondrial import inner membrane translocase subunit TIM50 antibody
      • TIM 50 antibody
      • TIM 50L antibody
      • TIM50 antibody
      • Tim50 like protein antibody
      • TIM50_HUMAN antibody
      • TIM50L antibody
      • TIMM 50 antibody
      • TIMM50 antibody
      • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) antibody
      • Translocase of inner mitochondrial membrane 50 homolog (yeast) antibody
      • Translocase of inner mitochondrial membrane 50 homolog antibody
      • Translocase of inner mitochondrial membrane 50, yeast, homolog of antibody
      see all

    Anti-TIMM50 antibody [EPR5785] images

    • All lanes : Anti-TIMM50 antibody [EPR5785] (ab109436) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-TIMM50 antibody [EPR5785] (ab109436)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application
    Western blot
    Sample
    Mouse Cell lysate - other (Homogenate of mouse brains, and mitochondria from)
    Gel Running Conditions
    Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol,10 min at 55C denaturation.)
    Loading amount
    20 µg
    Specification
    Homogenate of mouse brains, and mitochondria from
    Blocking step
    SEA Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 10 2017

    Thank you very much for your call yesterday.

    I've heard back from the lab, and the concentration of this lot is 0.932 mg/mL. The hybridoma cells are grown in serum-free media, so the supernatant does not contain any media.

    I hope th...

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