• Product nameAnti-TIMP1 antibody [2A5]
    See all TIMP1 primary antibodies
  • Description
    Mouse monoclonal [2A5] to TIMP1
  • SpecificityThis monoclonal antibody binds to human TIMP-1. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 72-82 of Human TIMP1.(Murray et al., 1998)

  • Positive control
    • Colon carcinoma.



Our Abpromise guarantee covers the use of ab2464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: Use at an assay dependent concentration. Perform antigen retrieval: microwave 7 min 950W or 20 min 800W in 0.01 M sodium citrate buffer pH 6.0.
    WB: Use at an assay dependent concentration.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
    • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • Post-translational
      The activity of TIMP1 is dependent on the presence of disulfide bonds.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Clgi antibody
      • Collagenase inhibitor antibody
      • Collagenase inhibitor, Human antibody
      • EPA antibody
      • EPO antibody
      • Erythroid Potentiating Activity antibody
      • Erythroid-potentiating activity antibody
      • Fibroblast collagenase inhibitor antibody
      • FLJ90373 antibody
      • HCI antibody
      • Human Collagenase Inhibitor antibody
      • Metalloproteinase inhibitor 1 antibody
      • Metalloproteinase inhibitor 1 precursor antibody
      • OTTHUMP00000023214 antibody
      • TIMP 1 antibody
      • TIMP antibody
      • TIMP metallopeptidase inhibitor 1 antibody
      • TIMP-1 antibody
      • Timp1 antibody
      • TIMP1 protein antibody
      • TIMP1_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 1 antibody
      • Tissue inhibitor of metalloproteinases 1 antibody
      • Tissue inhibitor of metalloproteinases antibody
      • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
      see all

    Anti-TIMP1 antibody [2A5] images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling TIMP1 with undiluted ab2464. Tissue sections were fixed in formaldehyde; heat-mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C, followed by incubation with Anti-TIMP1 antibody [2A5] (ab2464) for 12 hours at 20 °C  in PBS. A biotin conjugate goat anti-mouse secondary antibody was used at 1/100 dilution.

      See Abreview

    References for Anti-TIMP1 antibody [2A5] (ab2464)

    This product has been referenced in:

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (lung)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6,0
    Specification lung
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 27 2017

    Thank you for your enquiry. I have contacted the source of this antibody and unfortunately the concentration has not been determined. The starting dilution for IHC should be 1:2, in addition to serial dilutions to determine the optimal concentration...

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