![Sandwich ELISA - TIMP1 antibody [2E7.1] (ab28261) image](#TIMP1-Primary-antibodies-ab28261-3.jpg)
![Western blot - TIMP1 antibody [2E7.1] (ab28261) image](#ab28261_1.jpg)
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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP1 antibody [2E7.1]
See all TIMP1 products (17) ...
Description
Mouse monoclonal [2E7.1] to TIMP1
Specificity
This antibody binds to TIMP-1, but does not cross react with the other TIMP family members (TIMP-2, TIMP-3, TIMP-4). This product recognizes both native and reduced TIMP1.
Tested applications
Sandwich ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native protein (purified) of Human TIMP1.
Positive control
Cell media from human chondrosarcoma cells treated with TPA
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
2E7.1
Isotype
IgG1
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 1 µg/ml Can be paired for Sandwich ELISA with Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978). (For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab38978 as Detection.)
WB: 1/1000 - 1/5000.Predicted molecular weight: 23 kDa.(TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.)
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP01033
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor antibody
see all
Database links
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
Anti-TIMP1 antibody [2E7.1] images:
Sandwich ELISA - TIMP1 antibody [2E7.1] (ab28261)
![Sandwich ELISA - TIMP1 antibody [2E7.1] (ab28261)](/ps/datasheet/images/28/ab28261/TIMP1-Primary-antibodies-ab28261-3.jpg)
Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml.
Western blot - TIMP1 antibody [2E7.1] (ab28261)
![Western blot - TIMP1 antibody [2E7.1] (ab28261)](/ps/datasheet/Images/28/ab28261/ab28261_1.jpg)
All lanes : Anti-TIMP1 antibody [2E7.1] (ab28261)
Lane 1 : Human TIMP1
Lane 2 : Cell media from human chondrosarcoma (no treatment)
Lane 3 : Cell media from human chondrosarcoma (treated with TPA)
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
References for Anti-TIMP1 antibody [2E7.1] (ab28261)
ab28261 has not yet been referenced specifically in any publications.
Publishing research using ab28261? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"