The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain.
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Liu J et al. Homemade-device-induced negative pressure promotes wound healing more efficiently than VSD-induced positive pressure by regulating inflammation, proliferation and remodeling. Int J Mol Med39:879-888 (2017).
Read more (PubMed: 28290607) »
Zhong Y et al. Sclareol exerts anti-osteoarthritic activities in interleukin-1ß-induced rabbit chondrocytes and a rabbit osteoarthritis model. Int J Clin Exp Pathol8:2365-74 (2015).
Read more (PubMed: 26045743) »