The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 23 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TIMP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 5 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of Human TIMP1 (3.0 µg/ml).
sELISA: Use at a concentration of 0.5 - 2 µg/ml (using 100µl/well antibody solution).
Use a concentration of 0.5 - 2 µg/ml. (using 100µl/well antibody solution).
Use at an assay dependent concentration. PubMed: 22016393
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain.
Post-translational modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds.
Blocking - Anti-TIMP1 antibody (ab77847)Image adapted from Bernert B et al., J Biol Chem. 2011 Dec 9;286(49):42349-59. Epub 2011 Oct 20.Fig 6.; doi: 10.1074/jbc.M111.278598; December 9, 2011 The Journal of Biological Chemistry, 286, 42349-42359.
ab77847 was used to block TIMP1 in MDA-MB-231-BM cells.
Control = HAS2-expressing cells
C2-4 and C4-25 = HAS2 knockdown cells
References for Anti-TIMP1 antibody (ab77847)
This product has been referenced in:
Bernert B et al. Hyaluronan Synthase 2 (HAS2) Promotes Breast Cancer Cell Invasion by Suppression of Tissue Metalloproteinase Inhibitor 1 (TIMP-1). J Biol Chem286:42349-59 (2011).
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