Overview

  • Product name
    Anti-TIMP1 antibody [EPR1550]
    See all TIMP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1550] to TIMP1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TIMP1 aa 50-150.

  • Positive control
    • HL60+TPA, PBMC lysates; Human liver tissue; TIMP1 recombinant protein
  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/8000.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/250 - 1/500.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
    • Sequence similarities
      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • Post-translational
      modifications
      The activity of TIMP1 is dependent on the presence of disulfide bonds.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Clgi antibody
      • Collagenase inhibitor antibody
      • Collagenase inhibitor, Human antibody
      • EPA antibody
      • EPO antibody
      • Erythroid Potentiating Activity antibody
      • Erythroid-potentiating activity antibody
      • Fibroblast collagenase inhibitor antibody
      • FLJ90373 antibody
      • HCI antibody
      • Human Collagenase Inhibitor antibody
      • Metalloproteinase inhibitor 1 antibody
      • Metalloproteinase inhibitor 1 precursor antibody
      • OTTHUMP00000023214 antibody
      • TIMP 1 antibody
      • TIMP antibody
      • TIMP metallopeptidase inhibitor 1 antibody
      • TIMP-1 antibody
      • Timp1 antibody
      • TIMP1 protein antibody
      • TIMP1_HUMAN antibody
      • Tissue Inhibitor of Metalloproteinase 1 antibody
      • Tissue inhibitor of metalloproteinases 1 antibody
      • Tissue inhibitor of metalloproteinases antibody
      • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
      see all

    Images

    • All lanes : Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (purified)

      Lane 1 : Human prostate lysates at 20 µg
      Lane 2 : HT-1080 (Human fibrosarcoma epithelial cell) treated with 200ng/ml TPA for 24 hours whole cell lysates at 15 µg
      Lane 3 : Untreated with HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 23 kDa

      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling TIMP1 with purified ab109125 at 1:8000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • All lanes : Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (unpurified)

      Lane 1 : HL60+TPA cell lysates
      Lane 2 : PBMC cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 23 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling TIMP1 with purified ab109125 at 1:8000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Unpurified ab109125, at 1/250 dilution staining TIMP1 in paraffin-embedded Human liver tissue, by Immunohistochemistry.

    • Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (unpurified) + TIMP1 recombinant protein at 10 µg

      Predicted band size : 23 kDa

    References

    This product has been referenced in:
    • Wang C  et al. Etoposide Induces Apoptosis in Activated Human Hepatic Stellate Cells via ER Stress. Sci Rep 6:34330 (2016). WB ; Human . Read more (PubMed: 27680712) »
    • Lu X  et al. Evaluation of MMP-9 and MMP-2 and their suppressor TIMP-1 and TIMP-2 in adenocarcinoma of esophagogastric junction. Onco Targets Ther 9:4343-9 (2016). IHC . Read more (PubMed: 27486337) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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