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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP1 antibody - Loop 2
See all TIMP1 products (17) ...
Description
Rabbit polyclonal to TIMP1 - Loop 2
Specificity
This antibody recognizes reduced TIMP1, but does not bind as well to the non reduced protein. It also does not cross react with other TIMP family members (TIMP2, TIMP3, TIMP4).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on loop #2 and part of loop #1 of human TIMP1.
(Peptide available as ab41145.)
Positive control
Cell media from human chondrosarcoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> TIMP1 / TIMP2
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
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Western blot - TIMP1 antibody (ab38979)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 29 kDa when used against the reduced protein (predicted molecular weight: 23 kDa). Higher concentration of antibody may be needed for non human samples. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP1 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP01033
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Clgi antibody
- Collagenase inhibitor antibody
- EPA antibody
see all
Database links
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
Anti-TIMP1 antibody - Loop 2 images:
Western blot - TIMP1 antibody (ab38979)
All lanes : Anti-TIMP1 antibody - Loop 2 (ab38979) at 1/1000 dilution
Lane 1 : Cell media from human chondrosarcoma (no treatment)
Lane 2 : Cell media from human chondrosarcoma (treated with TPA)
Predicted band size : 23 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-TIMP1 antibody - Loop 2 (ab38979)
ab38979 has not yet been referenced specifically in any publications.
Publishing research using ab38979? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"