Overview

  • Product name
    Anti-TIMP2 antibody [3A4]
    See all TIMP2 primary antibodies
  • Description
    Mouse monoclonal [3A4] to TIMP2
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
  • Immunogen

    BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP 2.

  • Positive control
    • IHC-P: Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab1828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000.
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/100. PubMed: 24343133

Target

Images

  • Anti-TIMP2 antibody [3A4] (ab109708) at 5 µg/ml + Recombinant TIMP2 at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling TIMP2 with ab1828.

  • ICC/IF image of ab1828 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1828, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Liu J  et al. Homemade-device-induced negative pressure promotes wound healing more efficiently than VSD-induced positive pressure by regulating inflammation, proliferation and remodeling. Int J Mol Med 39:879-888 (2017). WB ; Rabbit . Read more (PubMed: 28290607) »
  • Zhao L  et al. Paired box 5 is a frequently methylated lung cancer tumour suppressor gene interfering ß-catenin signalling and GADD45G expression. J Cell Mol Med 20:842-54 (2016). WB ; Human . Read more (PubMed: 26843424) »

See all 21 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Ascending aorta)
Specification
Ascending aorta
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Mc Shen

Verified customer

Submitted Feb 05 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Tissue lysate - whole (ovaries)
Specification
ovaries
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Sep 30 2013

Thank you for contacting us.

The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

I am happy to provide discount code for others antib...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Astrocytes)
Loading amount
50 µg
Specification
Astrocytes
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
Yes - 0,1% Triton X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 29 2012


Yes you can use ab39315 as a positive control for western blotting with the anti-Timp2 antibody ab1828.
There is a note on the online product datasheet for ab39315 with some further details, which states a western blot "Produces a band of app...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal Muscle)
Loading amount
50 µg
Specification
Skeletal Muscle
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Steve John

Verified customer

Submitted Aug 07 2012

Thank you for contacting us. According to the alignment information that you have sent to me it looks like you should have success with using these antibodies with canine protein. I hope this information is helpful to you. Please do not hesitate to con...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caus...

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Thank you for taking the time to send me your protocols relating to this issue. I have been able to review them and agree that the problem here is the product. I would like to offer you free of charge replacements for ab1828 and ab61224. I may als...

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1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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