Anti-TIMP2 antibody [3A4] (Biotin) (ab79472)

Overview

  • Product nameAnti-TIMP2 antibody [3A4] (Biotin)
    See all TIMP2 primary antibodies
  • Description
    Mouse monoclonal [3A4] to TIMP2 (Biotin)
  • ConjugationBiotin
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
  • Immunogen

    Synthetic peptide from the N terminal region of Human TIMP2

  • Positive control
    • IHC-P: Human pancreatic or colon carcinoma itisue. WB: lysate from Human 35 cells. Conditioned, serum-free medium from TPA-treated HFL-1 cells; pancreatic or colon carcinoma tissue.
  • General notes


    May be used to evaluate the invasive nature of some tumor types and determine the tissue disatribution of TIMPs in other tumors such as adenomas or normal tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: 1/200 for 20-30 minutes at RT depending on the detection system used. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min prior to immunostaining.
    WB: Use at a concentration of 1 - 2 µg/ml for 2 hrs at RT. Predicted molecular weight: 24 kDa.

    Is unsuitable for IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-TIMP2 antibody [3A4] (Biotin) images

    • Anti-TIMP2 antibody [3A4] (Biotin) (ab79472) at 2 µg/ml + lysate from Human cells 35 at 20 µg

      Predicted band size : 24 kDa
      Observed band size : 24 kDa

    References for Anti-TIMP2 antibody [3A4] (Biotin) (ab79472)

    ab79472 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"