Overview

  • Product nameAnti-TIMP2 antibody
    See all TIMP2 primary antibodies
  • Description
    Rabbit polyclonal to TIMP2
  • Specificityab53730 detects endogenous levels of total TIMP2 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human TIMP2.

  • Positive control
    • A549 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 24 kDa).
ELISA 1/40000.

Target

  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSC 21K antibody
    • CSC-21K antibody
    • CSC21K antibody
    • Metalloproteinase inhibitor 2 antibody
    • Metalloproteinase inhibitor 2 precursor antibody
    • TIMP 2 antibody
    • TIMP metallopeptidase inhibitor 2 antibody
    • TIMP-2 antibody
    • TIMP2 antibody
    • TIMP2_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 2 antibody
    • Tissue inhibitor of metalloproteinases 2 antibody
    see all

Anti-TIMP2 antibody images

  • All lanes : Anti-TIMP2 antibody (ab53730) at 1/500 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with immunising peptide


    Predicted band size : 24 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)

References for Anti-TIMP2 antibody (ab53730)

This product has been referenced in:
  • Smith AC  et al. Synaptic plasticity mediating cocaine relapse requires matrix metalloproteinases. Nat Neurosci 17:1655-7 (2014). IHC ; Mouse . Read more (PubMed: 25326689) »
  • Fortin CF  et al. MT6-MMP is present in lipid rafts and faces inward in living human PMNs but translocates to the cell surface during neutrophil apoptosis. Int Immunol : (2010). Read more (PubMed: 20501611) »

See all 2 Publications for this product

Product Wall

Thank you for your patience.
The specific immunogen information is considered proprietary, but ab53730 binds to the NTR domain of the protein. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting us.
While the immunogen sequences for our antibodies are considered proprietary, we do provide general information about the immunogen range on our datasheets:
ab38972: Synthetic peptide based on the Human sequence. T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"