Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
Anti-TIMP2 antibody - Loop 3
See all TIMP2 products (16) ...
Description
Rabbit polyclonal to TIMP2 - Loop 3
Specificity
This antibody binds to TIMP2, but does not cross react with the other TIMP family members (TIMP1, TIMP3, TIMP4).
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Pig
Immunogen
Synthetic peptide based on the Human sequence. The antibody epitope is located in loop 3 of TIMP2.
(Peptide available as ab41150.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
-
Western blot - TIMP2 antibody (ab38972)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 24 kDa. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Higher concentration of antibody may be needed for non-human samples. When used against the reduced protein the antibody identifies a band at approximately 21 Kd. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Post-translational
modifications
The activity of TIMP2 is dependent on the presence of disulfide bonds.
Cellular localization
Secreted.
Target information above from: UniProt accessionP16035
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
Database links
- Entrez Gene: 282093 Cow
- Entrez Gene: 7077 Human
- Entrez Gene: 21858 Mouse
- Entrez Gene: 29543 Rat
- Omim: 188825 Human
- SwissProt: P16368 Cow
- SwissProt: P16035 Human
- SwissProt: P25785 Mouse
see all
Anti-TIMP2 antibody - Loop 3 images:
Western blot - TIMP2 antibody (ab38972)
All lanes : Anti-TIMP2 antibody - Loop 3 (ab38972)
Lane 1 : Human TIMP2
Lane 2 : Mouse TIMP2
Lane 3 : Cell media from human chondrosarcoma (untrested)
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-TIMP2 antibody - Loop 3 (ab38972)
ab38972 has not yet been referenced specifically in any publications.
Publishing research using ab38972? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"