The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use a concentration of 5 µg/ml. Predicted molecular weight: 24 kDa.
Use a concentration of 2 - 10 µg/ml.
Application notesIs unsuitable for ICC/IF or IP.
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Involvement in diseaseDefects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
ab58804 (1µg/ml) staining TIMP3 in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic/secreted staining of syncytiotrophoblasts. Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-TIMP3 antibody [136-13H4] (ab58804)
This product has been referenced in:
Rutnam ZJ et al. The pseudogene TUSC2P promotes TUSC2 function by binding multiple microRNAs. Nat Commun5:2914 (2014).
Read more (PubMed: 24394498) »
Yang X et al. Both mature miR-17-5p and passenger strand miR-17-3p target TIMP3 and induce prostate tumor growth and invasion. Nucleic Acids Res41:9688-704 (2013).
Read more (PubMed: 23990326) »