Synthetic peptide of TIMP3 based on loop #1 of the human TIMP3.
(Peptide available as ab41152.)
Our Abpromise guarantee covers the use of ab39184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/1000 - 1/5000. Detects a band of approximately 24, 30 kDa (predicted molecular weight: 24 kDa). Dilution optimised using Chromogenic detection.|
|IHC-P||Use at an assay dependent concentration. PubMed: 20448060
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab39184 staining TIMP3 in mouse fallopian tube tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBST (PBS / 0.1% v/v Triton X-100) and blocked with 3% donkey serum / 1% BSA in PBST for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 48 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Positive TIMP3 staining is seen in the extracellular space. The image shows DAPI counterstain, red field (autofluorescence), green field antibody staining and overlay.