Synthetic peptide of TIMP3 based on loop #1 of the human TIMP3.
(Peptide available as ab41152.)
Our Abpromise guarantee covers the use of ab39184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/1000 - 1/5000. Detects a band of approximately 24, 30 kDa (predicted molecular weight: 24 kDa). Dilution optimised using Chromogenic detection.|
|IHC-P||Use at an assay dependent concentration. PubMed: 20448060
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab39184 staining TIMP3 in mouse fallopian tube tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBST (PBS / 0.1% v/v Triton X-100) and blocked with 3% donkey serum / 1% BSA in PBST for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 48 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Positive TIMP3 staining is seen in the extracellular space. The image shows DAPI counterstain, red field (autofluorescence), green field antibody staining and overlay.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"