Anti-TIMP3 antibody (ab39184)
Key features and details
- Rabbit polyclonal to TIMP3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-TIMP3 antibody
See all TIMP3 primary antibodies -
Description
Rabbit polyclonal to TIMP3 -
Host species
Rabbit -
Specificity
ab39184 binds to TIMP3. It recognizes the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. Ab39184 does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4). -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide of TIMP3 based on loop #1 of the human TIMP3.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (2) |
Use a concentration of 1 - 5 µg/ml.
|
WB | (3) |
1/1000 - 1/5000. Detects a band of approximately 24, 30 kDa (predicted molecular weight: 24 kDa). Dilution optimised using Chromogenic detection.
|
Notes |
---|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
WB
1/1000 - 1/5000. Detects a band of approximately 24, 30 kDa (predicted molecular weight: 24 kDa). Dilution optimised using Chromogenic detection. |
Target
-
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. -
Involvement in disease
Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
Database links
- Entrez Gene: 7078 Human
- Entrez Gene: 21859 Mouse
- Omim: 188826 Human
- SwissProt: P35625 Human
- SwissProt: P39876 Mouse
- Unigene: 644633 Human
- Unigene: 714168 Human
- Unigene: 4871 Mouse
-
Alternative names
- HSMRK222 antibody
- K222 antibody
- K222TA2 antibody
see all
Images
-
All lanes : Anti-TIMP3 antibody (ab39184)
Lane 1 : Human TIMP3
Lane 2 : Mouse TIMP3
Lane 3 : crude control (preparation of extracellular matrix from BHK cells producing recombinant human TIMP-3)
Predicted band size: 24 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab39184 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39184, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
-
SDS download
-
Datasheet download
References (66)
ab39184 has been referenced in 66 publications.
- Park GY et al. Oxo-M and 4-PPBP Delivery via Multi-Domain Peptide Hydrogel Toward Tendon Regeneration. Front Bioeng Biotechnol 10:773004 (2022). PubMed: 35155388
- Tsuchiya K et al. m6A demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancer. Cancer Gene Ther 29:1355-1372 (2022). PubMed: 35318440
- Li L et al. Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A. Oxid Med Cell Longev 2022:9366494 (2022). PubMed: 35450405
- Hood EMS et al. Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts. STAR Protoc 3:101758 (2022). PubMed: 36227744
- Wu C et al. Tannic acid-loaded hydrogel coating endues polypropylene mesh with hemostatic and anti-inflammatory capacity for facilitating pelvic floor repair. Regen Biomater 9:rbac074 (2022). PubMed: 36267152