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Immunocytochemistry/ Immunofluorescence-TIMP3 antibody - Carboxyterminal end(ab39185)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-TIMP3 antibody - Carboxyterminal end(ab39185)
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Product Name
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TIMP3 antibody - Carboxyterminal end
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See all TIMP3 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TIMP3 - Carboxyterminal end
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Immunogen
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Synthetic peptide based on the carboxyterminal region of Human TIMP3.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody recognizes both the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. It does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4).
We have a range of domain specific antibodies for this target. For a full list please see all TIMP3 antibodies
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 - 1/5000. Detects a band of approximately 21 kDa when used under non reducing conditions (predicted molecular weight: 24 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Human amd mouse TIMP3.
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Cellular localization
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Secreted; extracellular space; extracellular matrix.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Inhibitors
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Cell Biology >> Apoptosis >> Extracellular Signals >> Granzymes
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Relevance
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The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightly bound inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knotlike structures. The N terminal region is necessary for inhibitory activity. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved to produce the mature protein. The C terminal regions are divergent and enhance the inhibition selectivity and binding efficiency. Although the TIMP proteins share high homology, following secretion they are localized extracellularly either in soluble form (TIMP1, TIMP2, and TIMP4) or bound to extracellular matrix components (TIMP3).
The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins).
Tissue Inhibitor of Metalloproteinase 3 (TIMP3) was first purified from chicken embryo fibroblasts and identified as ChIMP3. The human homologue of TIMP3, was originally detected as an inducible serum protein in WI-38 fibroblasts. The TIMP3 localization differs from that of the other three TIMPs, and is thought to be primarily deposited into the extracellular matrix (ECM). TIMP3 is insoluble, binds to the ECM associated with a variety of cell types, and is widely distributed throughout the body. TIMP3 shows 30% amino acid homology with TIMP1 and 38% homology with TIMP2. TIMP3 has been shown to promote the detachment of transformed cells from ECM and to accelerate morphological changes associated with cell transformation. Furthermore, up regulation of TIMP 3 has been associated with a block in the G1 phase of the cell cycle during differentiation of HL60 leukemia cells. The human TIMP3 gene has the chromosomal location of 22q12-22q13.
TIMP3 mRNA is highly expressed in placenta but is also found in the heart, kidney, lung, pancreas, uterus and skeletal muscle with low levels in the brain. In endometrium, TIMP3 is reported to be expressed in luminal epithelium, glands, stroma, endothelial cells and vascular smooth muscle cells. TIMP3 is also reported to be expressed by fibroblast-like cells in ulcerated intestinal wall
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TIMP3 antibody - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TIMP3 antibody - Carboxyterminal end images, references, products related to ab39185 and other tools.
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TIMP3 antibody - Carboxyterminal end images:
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 Immunocytochemistry/ Immunofluorescence-TIMP3 antibody - Carboxyterminal end(ab39185)
ICC/IF image of ab39185 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-TIMP3 antibody - Carboxyterminal end(ab39185)
Ab39185 staining Human normal placenta. Staining is localized to the cytoplasm or excreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for TIMP3 antibody - Carboxyterminal end (ab39185)
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This product has been used in:
Thomay AA et al. Disruption of interleukin-1 signaling improves the quality of wound healing. Am J Pathol 174:2129-36 (2009). WB; Mouse. PubMed: 19389930
If you publish research using ab39185 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to TIMP3
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