Anti-TIMP3 antibody - Carboxyterminal end (ab39185)


  • Product nameAnti-TIMP3 antibody - Carboxyterminal end
    See all TIMP3 primary antibodies
  • Description
    Rabbit polyclonal to TIMP3 - Carboxyterminal end
  • SpecificityThis antibody recognizes both the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. It does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4).
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide based on the carboxyterminal region of Human TIMP3.

  • Positive control
    • Human amd mouse TIMP3.



Our Abpromise guarantee covers the use of ab39185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 24 kDa). Used under non reducing conditions.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.


  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
  • Involvement in diseaseDefects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSMRK222 antibody
    • K222 antibody
    • K222TA2 antibody
    • Metalloproteinase inhibitor 3 antibody
    • MIG 5 protein antibody
    • MIG5 protein antibody
    • Protein MIG 5 antibody
    • Protein MIG-5 antibody
    • SFD antibody
    • Sorsby fundus dystrophy pseudoinflammatory antibody
    • TIMP 3 antibody
    • TIMP metallopeptidase inhibitor 3 antibody
    • TIMP-3 antibody
    • TIMP3 antibody
    • TIMP3_HUMAN antibody
    • Tissue Inhibitor of Metalloproteinase 3 antibody
    • Tissue inhibitor of metalloproteinases 3 antibody
    • Tissue inhibitor of metalloproteinases3 antibody
    see all

Anti-TIMP3 antibody - Carboxyterminal end images

  • ICC/IF image of ab39185 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab39185 staining Human normal placenta. Staining is localized to the cytoplasm or excreted.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-TIMP3 antibody - Carboxyterminal end (ab39185) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

References for Anti-TIMP3 antibody - Carboxyterminal end (ab39185)

This product has been referenced in:
  • Fiorentino L  et al. Loss of TIMP3 underlies diabetic nephropathy via FoxO1/STAT1 interplay. EMBO Mol Med 5:441-55 (2013). Mouse . Read more (PubMed: 23401241) »
  • Thomay AA  et al. Disruption of interleukin-1 signaling improves the quality of wound healing. Am J Pathol 174:2129-36 (2009). WB ; Mouse . Read more (PubMed: 19389930) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Casein in PBS as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
Sample Human Tissue sections (Kidney)
Specification Kidney
Permeabilization No
Fixative Formaldehyde

Dr. Francesca Conserva

Verified customer

Submitted Jul 12 2013

Thank you for contacting us.
I have checked, but unfortunately, for these antibodies we do not have more immunogen information than what is on the datasheet. The source where we receive these 3 antibodies from, considers any immunogen information ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies. The details you have kindly provided will enable us to investigate this case for you and also gives us vit...

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Thank you for providing the additional information and western blot data. It appears that ab39185 detects a protein of the expected molecular weight in the conditioned medium however the 50kDa band is seen in the OVC tissue extracts. And the ban...

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Thank you for your enquiry. Just a few additional questions regarding your western blot: 1. Have you used any other antibodies for western blotting with this ovarian cancer tissue extract? If so, what were the results? 2. The 50kD...

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