Overview

  • Product nameAnti-TIMP4 antibody
    See all TIMP4 primary antibodies
  • Description
    Rabbit polyclonal to TIMP4
  • SpecificityAb38987 recognises the carboxiterminal region of TIMP4. It does not react with other TIMPs.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Human
  • Immunogen

    Synthetic peptide based on the carboxyterminal region of human TIMP4.

    (Peptide available as ab41155.)

  • Positive control
    • Human TIMP4, cell media from human colorectal adenosarcoma and rat heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab38987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).

Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates.

Predicted molecular weight: 26 kDa. When used against the reduced protein, ab38987 identifies bands at 23 and 29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively.

Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection.

Target

  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
  • Tissue specificityAbundant in heart and present at low levels in many other tissues.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Metalloproteinase inhibitor 4 antibody
    • TIMP 4 antibody
    • TIMP metallopeptidase inhibitor 4 antibody
    • TIMP-4 antibody
    • Timp4 antibody
    • TIMP4_HUMAN antibody
    • Tissue inhibitor of metalloproteinase 4 antibody
    • Tissue inhibitor of metalloproteinases 4 antibody
    see all

Anti-TIMP4 antibody images

  • All lanes : Anti-TIMP4 antibody (ab38987) at 1/1000 dilution

    Lane 1 : human TIMP4
    Lane 2 : cell media from human colorectal adenosarcoma
    Lane 3 : rat heart


    Predicted band size : 26 kDa
    Observed band size : 25,45,66,97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,23 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.

References for Anti-TIMP4 antibody (ab38987)

This product has been referenced in:
  • Horn MA  et al. Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged. J Mol Cell Cardiol 53:82-90 (2012). WB ; Sheep . Read more (PubMed: 22516365) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Sheep Tissue lysate - whole (Heart)
Loading amount 25 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jul 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"