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Product name Anti-TIMP4 antibody See all TIMP4 primary antibodies
Rabbit polyclonal to TIMP4
Specificity ab58425 detects endogenous levels of total TIMP4 protein.
Tested applications Suitable for:
IHC-Fr, ELISA, IHC-P, WB more details
Mouse, Rat, Human
Synthetic peptide derived from C terminal residues of human TIMP4
IHC-P: Human lung carcinoma tissue.
WB: NIH3T3 cell extracts.
Storage instructions Store at -20°C. Stable for 12 months at -20°C
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab58425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23525608
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Tissue specificity Abundant in heart and present at low levels in many other tissues.
Sequence similarities Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain.
Cellular localization Secreted.
Information by UniProt
Metalloproteinase inhibitor 4 antibody
TIMP 4 antibody
TIMP metallopeptidase inhibitor 4 antibody
Anti-TIMP4 antibody images
Western blot - TIMP4 antibody (ab58425)
All lanes : Anti-TIMP4 antibody (ab58425) at 1/500 dilution Lane 1 : NIH3T3 cell extract Lane 2 : NIH3T3 cell extract with immunising peptide Predicted band size : 26 kDa Observed band size : 26 kDa
Immunohistochemistry (Paraffin-embedded sections) - TIMP4 antibody (ab58425)
ab58425, at 1/50 dilution, staining TIMP4 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunising peptide.
References for Anti-TIMP4 antibody (ab58425)
This product has been referenced in:
et al. Hyperhomocysteinemia during aortic aneurysm, a plausible role of epigenetics. Int J Physiol Pathophysiol Pharmacol 5:32-42 (2013).
Read more (PubMed: 23525608) »
et al. Maternal hypoxia alters matrix metalloproteinase expression patterns and causes cardiac remodeling in fetal and neonatal rats. Am J Physiol Heart Circ Physiol 301:H2113-21 (2011).
Read more (PubMed: 21856922) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"