Overview

  • Product nameAnti-TIMP4 antibody [SB30c]
    See all TIMP4 primary antibodies
  • Description
    Mouse monoclonal [SB30c] to TIMP4
  • Tested applicationsSuitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    Recombinant full length protein, Human TIMP4

  • Positive control
    • Human pancreatic carcinoma cells and cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab51207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-Fr
ICC/IF
  • Application notesICC/IF: Use at a concentration of 5 µg/ml. (Methanol fixation).
    IHC-Fr: Use at a concentration of 5 µg/ml.
    WB: Use at an assay dependent dilution. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
    • Tissue specificityAbundant in heart and present at low levels in many other tissues.
    • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Metalloproteinase inhibitor 4 antibody
      • TIMP 4 antibody
      • TIMP metallopeptidase inhibitor 4 antibody
      • TIMP-4 antibody
      • Timp4 antibody
      • TIMP4_HUMAN antibody
      • Tissue inhibitor of metalloproteinase 4 antibody
      • Tissue inhibitor of metalloproteinases 4 antibody
      see all

    Anti-TIMP4 antibody [SB30c] images

    • Immunocytochemical analysis of methanol fixed human pancreatic carcinoma cells labelling TIMP-4 with ab51207 at 5 ug/ml.

    • Anti-TIMP4 antibody [SB30c] (ab51207) at 2 µg + pancreatic cancer cell lysate

      Secondary
      Goat anti-mouse IgG2a and conjugated to HRP (human adsorbed) at 1/4000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa

    • Developed using the ECL technique

      Predicted band size : 26 kDa


      Exposure time : 10 seconds

      Image courtesy of an anonymous Abreview.

      All nine lanes are cardiac tissue samples from different rabbit hearts.
      ab51207 used at 0.5µg/ml.
      Whole tissue lysate loaded at 40µg.
      Secondary used was a HRP conjugated donkey anti-mouse polyclonal used at a 1/4000 dilution.

      See Abreview

    References for Anti-TIMP4 antibody [SB30c] (ab51207)

    This product has been referenced in:
    • Zambirinis CP  et al. TLR9 ligation in pancreatic stellate cells promotes tumorigenesis. J Exp Med 212:2077-94 (2015). WB ; Human . Read more (PubMed: 26481685) »
    • Alarab M  et al. Expression of Extracellular Matrix-Remodeling Proteins Is Altered in Vaginal Tissue of Premenopausal Women With Severe Pelvic Organ Prolapse. Reprod Sci N/A:N/A (2013). WB, IHC-Fr ; Human . Read more (PubMed: 24343133) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Sheep Tissue lysate - whole (Placenta, caruncle)
    Gel Running Conditions Non-reduced Denaturing (10% PAG)
    Loading amount 10 µg
    Specification Placenta, caruncle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Mr. Jeremy Gingrich

    Verified customer

    Submitted Feb 05 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Glioblastoma primary cells)
    Loading amount 25 µg
    Specification Glioblastoma primary cells
    Gel Running Conditions Reduced Denaturing (gradient gel 5-15%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 20 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rabbit Tissue lysate - whole (heart)
    Loading amount 40 µg
    Specification heart
    Gel Running Conditions Non-reduced Denaturing (12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2012

    I am very pleased to hear you would like to accept our offer and test ab47709, ab51207, and ab79188 inrabbit. This code will give you 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please submit an Abreview for each an...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"