Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Overview
Product name
TIMP4 protein (Human)
See all TIMP4 products (5) ...
Protein description
Recombinant full length Human TIMP4 protein purified from transfected human kidney cells.
Properties
Purity
>95% by SDS-PAGE
Purification notes
This protein is free of contaminating MMPs or other known TIMPs.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mM MES, 50% Glycerol, 0.02% Brij 35, 300mM Sodium chloride, pH 5.0
Concentration
Concentration information loading...
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Metalloprotease inhibitors >> TIMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> TIMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> TIMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> TIMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Inhib: Use at an assay dependent dilution. (PubMed: 20422465Reverses the attenuation of votage-induced primary cardiomyocyte contraction induced by MMP9.)
WB: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
Protein info
Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Tissue specificity
Abundant in heart and present at low levels in many other tissues.
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ99727
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for TIMP4 protein (Human) (ab39319)
This product has been referenced in:
- Mishra PKet al. MMP-9 gene ablation and TIMP-4 mitigate PAR-1-mediated cardiomyocyte dysfunction: a plausible role of dicer and miRNA. Cell Biochem Biophys 57:67-76 (2010). Inhibition Assay.Read more (PubMed: 20422465) »
See 1 publication for this product
Publishing research using ab39319? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"