Overview

  • Product nameAnti-Tin2 antibody [59B388]
    See all Tin2 primary antibodies
  • Description
    Mouse monoclonal [59B388] to Tin2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    VAPGLVRYRHHERLC

    , corresponding to amino acids 44-58 of Human Tin2.

  • Positive control
    • 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesWB: Use at a concentration of 1 - 2 ug/ml. Detects a band of approximately 40 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Plays a role in shelterin complex assembly.
    • Tissue specificityDetected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Involvement in diseaseDefects in TINF2 are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM:127550]; also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
      Defects in TINF2 are a cause of retinopathy exudative with bone marrow failure (ERBMF) [MIM:268130]; also known as Revesz syndrome. ERBMF is characterized by bilateral exudative retinopathy, bone marrow hypoplasia, nail dystrophy, fine hair, cerebellar hypoplasia, and growth retardation.
    • DomainThe TBM domain mediates interaction with TERF1.
    • Cellular localizationNucleus. Chromosome > telomere. Associated with telomeres.
    • Information by UniProt
    • Database links
    • Alternative names
      • AW552114 antibody
      • D14Wsu146e antibody
      • DKCA3 antibody
      • MGC94711 antibody
      • TERF 1 (TRF 1) interacting nuclear factor 2 antibody
      • TERF 1 interacting nuclear factor 2 antibody
      • TERF1 (TRF1) interacting nuclear factor 2 antibody
      • TERF1 interacting nuclear factor 2 antibody
      • TERF1-interacting nuclear factor 2 antibody
      • Tin 2 antibody
      • TIN2 antibody
      • TINF 2 antibody
      • Tinf2 antibody
      • TINF2_HUMAN antibody
      • TRF 1 interacting nuclear factor 2 antibody
      • TRF1 interacting nuclear factor 2 antibody
      • TRF1-interacting nuclear protein 2 antibody
      see all

    Anti-Tin2 antibody [59B388] images

    • All lanes : Anti-Tin2 antibody [59B388] (ab13791) at 2 µg/ml

      Lane 1 : Daudi cell lysate
      Lane 2 : 3T3 cell lysate

    References for Anti-Tin2 antibody [59B388] (ab13791)

    This product has been referenced in:
    • Choi KH  et al. Characterization of the DNA binding specificity of Shelterin complexes. Nucleic Acids Res : (2011). WB, EMSA ; Human . Read more (PubMed: 21852327) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Human Cell (293T)
    Specification 293T
    Permeabilization Yes - Triton 1%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 02 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step FBS / BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample Mouse Tissue sections (Mouse 18dpc)
    Specification Mouse 18dpc
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 15 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (mMEF)
    Loading amount 50 µg
    Specification mMEF
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 18 2012

    Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antibody. I have read your customer's technical questionnaire and I have a few comments. The conditions that they are employing are very similar...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"