Overview

  • Product nameAnti-TIP120A antibody
    See all TIP120A primary antibodies
  • Description
    Rabbit polyclonal to TIP120A
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1180 and 1230 of Human TIP120A (NP_060918.2).

  • Positive control
    • 293T, NIH3T3 and HeLa cell lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionEnhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins.
  • Sequence similaritiesBelongs to the CAND family.
    Contains 27 HEAT repeats.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAND 1 antibody
    • Cand1 antibody
    • CAND1_HUMAN antibody
    • Cullin associated and neddylation dissociated 1 antibody
    • Cullin associated and neddylation dissociated protein 1 antibody
    • Cullin associated NEDD8 dissociated protein 1 antibody
    • Cullin-associated and neddylation-dissociated protein 1 antibody
    • Cullin-associated NEDD8-dissociated protein 1 antibody
    • DKFZP434M1414 antibody
    • FLJ10114 antibody
    • FLJ10929 antibody
    • FLJ38691 antibody
    • FLJ90441 antibody
    • KIAA0829 antibody
    • p120 CAND1 antibody
    • TATA binding protein interacting protein 120A antibody
    • TBP interacting protein antibody
    • TBP interacting protein of 120 kDa A antibody
    • TBP Interacting Protein TIP120A antibody
    • TBP-interacting protein 120A antibody
    • TBP-interacting protein of 120 kDa A antibody
    • TIP120 antibody
    • TIP120 protein antibody
    see all

Anti-TIP120A antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse hybridoma tumor (right) tissues labelling TIP120A with ab101987 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TIP120A antibody (ab101987) at 0.1 µg

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg


    Predicted band size : 136 kDa
  • Detection of TIP120A by Western blot of Immunprecipitate.
    ab101987, at 1 µg/ml, staining TIP120A in HeLa whole cell lysate immunoprecipitated using ab101987 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-TIP120A antibody (ab101987)

ab101987 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"