Overview

  • Product nameAnti-TIP30 antibody
    See all TIP30 primary antibodies
  • Description
    Rabbit polyclonal to TIP30
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Chimpanzee, Spider Monkey
  • Immunogen

    A region within a synthetic peptide: EILEQGLFSK VTLIGRRKLT FDEEAYKNVN QEVVDFEKLD DYASAFQGHD, corresponding to N terminal amino acids 37-86 of Human TIP30

  • Positive control
    • Jurkat cell lysate (western blot), human liver tissue slide (IHC-P).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab22841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 19104151

Target

  • FunctionOxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.
  • Tissue specificityUbiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.
  • DomainUnique C-terminus confers high proteasome-dependent instability to isoform 2.
  • Cellular localizationCytoplasm. Nucleus envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • 30 kDa HIV 1 TAT interacting protein antibody
    • 30 kDa HIV-1 TAT-interacting protein antibody
    • 30 kDa HIV1 TAT interacting protein antibody
    • CC3 antibody
    • FLJ26963 antibody
    • HIV 1 Tat interacting protein 2 30kDa antibody
    • HIV 1 Tat interacting protein 2 antibody
    • HIV-1 TAT-interactive protein 2 antibody
    • HTAI2_HUMAN antibody
    • HTATIP 2 antibody
    • HTATIP2 antibody
    • Oxidoreductase HTATIP2 antibody
    • SDR44U1 antibody
    • Short chain dehydrogenase/reductase family 44U member 1 antibody
    • Tat interacting protein (30kDa) antibody
    see all

Anti-TIP30 antibody images

  • Anti-TIP30 antibody (ab22841) (0.5 - 1.0 ug/ml) + Jurkat cell lysate

    Predicted band size : 27 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling TIP30 with ab22841 at a concentration of 4-8µg/ml. Positive labelled hepatocytes are indicated (arrows). Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling TIP30 with ab22841 at a concentration of 4-8µg/ml. Positive labelled alveolar cells are indicated (arrows). Magnification: 400X.

References for Anti-TIP30 antibody (ab22841)

This product has been referenced in:
  • Nakahara J  et al. Abnormal expression of TIP30 and arrested nucleocytoplasmic transport within oligodendrocyte precursor cells in multiple sclerosis. J Clin Invest 119:169-81 (2009). IHC-Fr ; Human . Read more (PubMed: 19104151) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"