Overview

  • Product nameAnti-TIP30 antibody
    See all TIP30 primary antibodies
  • Description
    Rabbit polyclonal to TIP30
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human TIP30.

    (Peptide available as ab74501.)

  • Positive control
    • This antibody gave a positive signal in SW480 Whole Cell Lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 27 kDa).

Target

  • FunctionOxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.
  • Tissue specificityUbiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.
  • DomainUnique C-terminus confers high proteasome-dependent instability to isoform 2.
  • Cellular localizationCytoplasm. Nucleus envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • 30 kDa HIV 1 TAT interacting protein antibody
    • 30 kDa HIV-1 TAT-interacting protein antibody
    • 30 kDa HIV1 TAT interacting protein antibody
    • CC3 antibody
    • FLJ26963 antibody
    • HIV 1 Tat interacting protein 2 30kDa antibody
    • HIV 1 Tat interacting protein 2 antibody
    • HIV-1 TAT-interactive protein 2 antibody
    • HTAI2_HUMAN antibody
    • HTATIP 2 antibody
    • HTATIP2 antibody
    • Oxidoreductase HTATIP2 antibody
    • SDR44U1 antibody
    • Short chain dehydrogenase/reductase family 44U member 1 antibody
    • Tat interacting protein (30kDa) antibody
    see all

Anti-TIP30 antibody images

  • Anti-TIP30 antibody (ab71752) at 1 µg/ml + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-TIP30 antibody (ab71752)

ab71752 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Nov 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"