Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TIP30 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary. Database link: Q9BUP3
Human fetal liver tissue lysate; HeLa, A431, A549 and SW480 cell lysates
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC/IF or IHC-P.
Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.
Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.
Unique C-terminus confers high proteasome-dependent instability to isoform 2.
Western blot analysis on immunoprecipitation pellet from A431 cell lysate labeling TIP30 with ab181378 at 1/10 dilution (Lane 1). Lane 2 is 1XPBS (negative control). Detection with HRP-conjugated anti-rabbit IgG, detecting the non-reduced form of rabbit IgG.