Recombinant
RabMAb

Anti-TIP30 antibody [EPR13185] (ab181378)

Overview

  • Product name
    Anti-TIP30 antibody [EPR13185]
    See all TIP30 primary antibodies
  • Description
    Rabbit monoclonal [EPR13185] to TIP30
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TIP30 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BUP3

  • Positive control
    • Human fetal liver tissue lysate; HeLa, A431, A549 and SW480 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 27 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.
    • Tissue specificity
      Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.
    • Domain
      Unique C-terminus confers high proteasome-dependent instability to isoform 2.
    • Cellular localization
      Cytoplasm. Nucleus envelope.
    • Information by UniProt
    • Database links
    • Alternative names
      • 30 kDa HIV 1 TAT interacting protein antibody
      • 30 kDa HIV-1 TAT-interacting protein antibody
      • 30 kDa HIV1 TAT interacting protein antibody
      • CC3 antibody
      • FLJ26963 antibody
      • HIV 1 Tat interacting protein 2 30kDa antibody
      • HIV 1 Tat interacting protein 2 antibody
      • HIV-1 TAT-interactive protein 2 antibody
      • HTAI2_HUMAN antibody
      • HTATIP 2 antibody
      • HTATIP2 antibody
      • Oxidoreductase HTATIP2 antibody
      • SDR44U1 antibody
      • Short chain dehydrogenase/reductase family 44U member 1 antibody
      • Tat interacting protein (30kDa) antibody
      see all

    Images

    • All lanes : Anti-TIP30 antibody [EPR13185] (ab181378) at 1/10000 dilution

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 27 kDa
    • Western blot analysis on immunoprecipitation pellet from A431 cell lysate labeling TIP30 with ab181378 at 1/10 dilution (Lane 1). Lane 2 is 1XPBS (negative control).  Detection with HRP-conjugated anti-rabbit IgG, detecting the non-reduced form of rabbit IgG.

    • Flow cytometric analysis of permeabilized HeLa cells (red) labeling TIP30 with ab181378 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

    References

    ab181378 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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