The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
FunctionRequired for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.
Sequence similaritiesBelongs to the CSM3 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Immunocytochemistry/ Immunofluorescence - TIPIN antibody (ab57488)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab57488 (1/200) detecting TIPIN in assynchronous HeLa cells (green). Cells were fixed in Paraformadehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.