Overview

  • Product nameAnti-TIPIN antibody
    See all TIPIN primary antibodies
  • Description
    Mouse monoclonal to TIPIN
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-302 of Human TIPIN

Properties

Applications

Our Abpromise guarantee covers the use of ab57488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
ICC/IF 1/200.

Target

  • FunctionRequired for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light.
  • Sequence similaritiesBelongs to the CSM3 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20516 antibody
    • TIMELESS interacting protein antibody
    • TIMELESS-interacting protein antibody
    • TIPIN antibody
    • TIPIN_HUMAN antibody
    see all

Anti-TIPIN antibody images

  • ab57488 (1/200) detecting TIPIN in assynchronous HeLa cells (green). Cells were fixed in Paraformadehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • All lanes : Anti-TIPIN antibody (ab57488) at 1/500 dilution

    Lane 1 : 20 µg Human HCT116 (colorectal) whole cell lysate
    Lane 2 : 20 µg Human HCT116 (colorectal) whole cell lysate treated with GAPD siRNA (3 days)
    Lane 3 : 20 µg Human HCT116 (colorectal) whole cell lysate treated with TIPIN siRNA (3 days)

    Secondary
    Goat anti-Mouse conjugated to HRP at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa (possible non-specific binding).

    This image is courtesy of an Abreview submitted by Dr Kirk McManus

    See Abreview

References for Anti-TIPIN antibody (ab57488)

ab57488 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Fibroblast)
Loading amount 300 µg
Specification Fibroblast
Treatment 100nM siRNA against Tipin/Timeless
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116 (colorectal))
Specification HCT116 (colorectal)
Treatment TIPIN siRNA
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Kirk McManus

Verified customer

Submitted Jul 15 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted May 12 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (fibroblast (XP30RO))
Loading amount 50 µg
Specification fibroblast (XP30RO)
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"