Overview

  • Product nameAnti-TIPRL antibody
    See all TIPRL primary antibodies
  • Description
    Rabbit polyclonal to TIPRL
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 225 and the C-terminus (residue 272) of human TIPRL (NP_690866.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab70795 was affinity purified using an epitope specific to TIPRL immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMay be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.
  • Sequence similaritiesBelongs to the TIP41 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1810011K17Rik antibody
    • dJ69E11.3 antibody
    • MGC3794 antibody
    • Putative MAPK activating protein PM10 antibody
    • Putative MAPK-activating protein PM10 antibody
    • RP1-69E11.1 antibody
    • Tip antibody
    • TIP41 antibody
    • TIP41-like protein antibody
    • tiprl antibody
    • TIPRL_HUMAN antibody
    • TOR Signaling Pathway Regulator like antibody
    • Type 2A-interacting protein antibody
    see all

Anti-TIPRL antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling TIPRL with ab70795 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TIPRL antibody (ab70795) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml
    Lane 4 : 293T whole cell lysate at 50 µg/ml
    Lane 5 : NIH3T3 whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 32 kDa


    Exposure time : 3 seconds
  • Detection of TIPRL by Western Blot of Immunoprecipitate.
    ab70795 at 1µg/ml staining TIPRL in HeLa whole cell lysate immunoprecipitated using ab70795 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References for Anti-TIPRL antibody (ab70795)

ab70795 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70795.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"