Overview

  • Product name
  • Description
    Rabbit polyclonal to TIPRL
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 200 and 250 of human TIPRL (NP_690866.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab71974 was affinity purified using an epitope specific to TIPRL immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 32 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.
    • Sequence similarities
      Belongs to the TIP41 family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1810011K17Rik antibody
      • dJ69E11.3 antibody
      • MGC3794 antibody
      • Putative MAPK activating protein PM10 antibody
      • Putative MAPK-activating protein PM10 antibody
      • RP1-69E11.1 antibody
      • Tip antibody
      • TIP41 antibody
      • TIP41-like protein antibody
      • tiprl antibody
      • TIPRL_HUMAN antibody
      • TOR signaling pathway regulator antibody
      • TOR Signaling Pathway Regulator like antibody
      • Type 2A-interacting protein antibody
      see all

    Images

    • All lanes : Anti-TIPRL antibody (ab71974) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 32 kDa
      Observed band size : 29 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    References

    ab71974 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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