Anti-TIRAP antibody [20D1055] (ab13684)
Key features and details
- Mouse monoclonal [20D1055] to TIRAP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-TIRAP antibody [20D1055]
See all TIRAP primary antibodies -
Description
Mouse monoclonal [20D1055] to TIRAP -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HUVEC cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
20D1055 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 32-37 kDa (predicted molecular weight: 26.5 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 32-37 kDa (predicted molecular weight: 26.5 kDa). |
Target
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Relevance
The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 114609 Human
- GenBank: AF378129 Human
- GenBank: AF378130 Human
- Omim: 606252 Human
- SwissProt: P58753 Human
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Alternative names
- Adapter protein wyatt antibody
- FLJ42305 antibody
- MAL antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab13684 has been referenced in 2 publications.
- Guo Q et al. Overexpression of oncostatin M receptor regulates local immune response in glioblastoma. J Cell Physiol N/A:N/A (2019). PubMed: 30693511
- Jeyaseelan S et al. Induction of CXCL5 during inflammation in the rodent lung involves activation of alveolar epithelium. Am J Respir Cell Mol Biol 32:531-9 (2005). WB ; Mouse . PubMed: 15778492