Overview

  • Product nameAnti-TIRAP antibody [20D1055]
    See all TIRAP primary antibodies
  • Description
    Mouse monoclonal [20D1055] to TIRAP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HUVEC cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 32-37 kDa (predicted molecular weight: 26.5 kDa).

Target

  • RelevanceThe Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Adapter protein wyatt antibody
    • FLJ42305 antibody
    • MAL antibody
    • MyD88 adapter like protein antibody
    • TIR domain containing adapter protein antibody
    • Toll interleukin 1 receptor (TIR) domain containing adaptor protein antibody
    • Toll interleukin 1 receptor domain containing adapter protein antibody
    • Toll like receptor adaptor protein antibody
    • Wyatt antibody
    see all

Anti-TIRAP antibody [20D1055] images



  • Predicted band size : 26.5 kDa


    Western blot analysis of TIRAP using ab13684 at 1:100 on 20 µgs of HUVEC cell lysate.

References for Anti-TIRAP antibody [20D1055] (ab13684)

This product has been referenced in:
  • Jeyaseelan S  et al. Induction of CXCL5 during inflammation in the rodent lung involves activation of alveolar epithelium. Am J Respir Cell Mol Biol 32:531-9 (2005). WB ; Mouse . Read more (PubMed: 15778492) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab13684.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"