Overview

  • Product name
  • Description
    Goat polyclonal to TIRAP
  • Specificity
    This protein is expected to recognise isoform b only (as represented by NP_683708).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    EGEGERDSATVSDL

    , corresponding to C terminal amino acids 221-234 of Human TIRAP

Properties

Applications

Our Abpromise guarantee covers the use of ab1349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent concentration.

Peptide ELISA: Antibody detection limit dilution 1:16,000.

Target

  • Relevance
    The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Adapter protein wyatt antibody
    • FLJ42305 antibody
    • MAL antibody
    • MyD88 adapter like protein antibody
    • TIR domain containing adapter protein antibody
    • Toll interleukin 1 receptor (TIR) domain containing adaptor protein antibody
    • Toll interleukin 1 receptor domain containing adapter protein antibody
    • Toll like receptor adaptor protein antibody
    • Wyatt antibody
    see all

Images

  • Anti-TIRAP antibody (ab1349) at 1 µg/ml + Human Liver lysate (35ug protein in RIPA buffer)

    Predicted band size : 24 kDa

References

ab1349 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab1349.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up