Overview

  • Product nameAnti-TIRAP antibody
    See all TIRAP primary antibodies
  • Description
    Rabbit polyclonal to TIRAP
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide. 15 amino acids near the C-terminus of mouse TIRAP (GenBank accession no.AAL05628).

  • Positive control
    • Human heart cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab17218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 34 kDa.Can be blocked with Mouse TIRAP peptide (ab39884).
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 2 µg/ml.

Target

  • RelevanceThe Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Adapter protein wyatt antibody
    • FLJ42305 antibody
    • MAL antibody
    • MyD88 adapter like protein antibody
    • TIR domain containing adapter protein antibody
    • Toll interleukin 1 receptor (TIR) domain containing adaptor protein antibody
    • Toll interleukin 1 receptor domain containing adapter protein antibody
    • Toll like receptor adaptor protein antibody
    • Wyatt antibody
    see all

Anti-TIRAP antibody images

  • Lane 1 : Anti-TIRAP antibody (ab17218) at 0.5 µg/ml
    Lane 2 : Anti-TIRAP antibody (ab17218) at 1 µg/ml
    Lane 3 : Anti-TIRAP antibody (ab17218) at 2 µg/ml

    Lane 1 : heart cell lysate
    Lane 2 : heart cell lysate
    Lane 3 : heart cell lysate


    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab17218 at 2µg/ml staining TIRAP in human heart cells by IHC

  • ab17218 staining TIRAP in a PMA-treated Human THP-1 cell by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde for 30 minutes and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. The secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit polyclonal, diluted 1/500 (green). Other stains used were Alexa Fluor 595-conjugated wheat germ agglutinin (red) and Nuclei were stained with Hoechst 33258 (blue)

    See Abreview

References for Anti-TIRAP antibody (ab17218)

This product has been referenced in:
  • Sakuma C  et al. Specific binding of the WASP N-terminal domain to Btk is critical for TLR2 signaling in macrophages. Mol Immunol 63:328-36 (2015). Read more (PubMed: 25213142) »
  • Sakaguchi M  et al. DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes. J Biol Chem 289:23389-402 (2014). WB ; Human . Read more (PubMed: 25002577) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Rhesus monkey Tissue sections (Colon)
Specification Colon
Permeabilization Yes - 0.1% Triton-x-100
Fixative Formaldehyde
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Abcam user community

Verified customer

Submitted Jun 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (THP-1)
Specification THP-1
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Aug 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"