• Product nameAnti-TIRAP antibody
    See all TIRAP primary antibodies
  • Description
    Goat polyclonal to TIRAP
  • SpecificityThis antibody is expected to recognize both reported isoforms (NP_001034750.1 and NP_683708.1).
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 27-41 of Human TIRAP

  • Positive control
    • K562 cell lysate.


Associated products


Our Abpromise guarantee covers the use of ab47986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesPeptide ELISA: Antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 24 kDa observed in lysates of Human myeloid erythroblast call line K562 (predicted molecular weight: 24 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • Adapter protein wyatt antibody
      • FLJ42305 antibody
      • MAL antibody
      • MyD88 adapter like protein antibody
      • TIR domain containing adapter protein antibody
      • Toll interleukin 1 receptor (TIR) domain containing adaptor protein antibody
      • Toll interleukin 1 receptor domain containing adapter protein antibody
      • Toll like receptor adaptor protein antibody
      • Wyatt antibody
      see all

    Anti-TIRAP antibody images

    • Anti-TIRAP antibody (ab47986) at 0.1 µg/ml + K562 cell lysate (35µg protein in RIPA buffer).

      Predicted band size : 24 kDa
      Observed band size : 24 kDa

    References for Anti-TIRAP antibody (ab47986)

    ab47986 has not yet been referenced specifically in any publications.

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