Overview

  • Product nameAnti-TIRAP antibody
    See all TIRAP primary antibodies
  • Description
    Rabbit polyclonal to TIRAP
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human TIRAP

  • Positive control
    • Human skeletal muscle tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab78070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.
ELISA Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

  • RelevanceThe Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TIRAP (TIR domain-containing adaptor protein) is an adaptor protein used by TLR4. Blocking TIRAP inhibits TLR4-mediated signaling events, including DC maturation and cytokine production. Function: Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6. Tissue specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain. Post-translational modification: Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Sequence similarities: Contains 1 TIR domain.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Adapter protein wyatt antibody
    • FLJ42305 antibody
    • MAL antibody
    • MyD88 adapter like protein antibody
    • TIR domain containing adapter protein antibody
    • Toll interleukin 1 receptor (TIR) domain containing adaptor protein antibody
    • Toll interleukin 1 receptor domain containing adapter protein antibody
    • Toll like receptor adaptor protein antibody
    • Wyatt antibody
    see all

Anti-TIRAP antibody images

  • ab78070, at 10 µg/ml, staining human TIRAP in skeletal muscle, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

References for Anti-TIRAP antibody (ab78070)

This product has been referenced in:
  • Yew KH  et al. Human cytomegalovirus induces TLR4 signaling components in monocytes altering TIRAP, TRAM and downstream interferon-beta and TNF-alpha expression. PLoS One 7:e44500 (2012). WB . Read more (PubMed: 22970235) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"