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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.125 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent dilution. PubMed: 20691152
Function Probable regulatory protein involved in regulating the response to growth factors.
Sequence similarities Contains 2 C3H1-type zinc fingers.
Cellular localization Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
Information by UniProt
Butyrate response factor 1 antibody
Anti-TIS11B antibody images
Western blot - Anti-TIS11B antibody (ab42473)
Anti-TIS11B antibody (ab42473) at 0.125 µg/ml (5% skimmed milk / PBS) + 10µg Human placenta whole cell lysate Secondary HRP conjugated anti-rabbit at 1/50000 dilution Predicted band size : 36 kDa Observed band size : 30 kDa ( why is the actual band size different from the predicted?) Gel concentration 12%.
References for Anti-TIS11B antibody (ab42473)
This product has been referenced in:
et al. Ectopic over-expression of tristetraprolin in human cancer cells promotes biogenesis of let-7 by down-regulation of Lin28. Nucleic Acids Res : (2011).
Read more (PubMed: 22210895) »
et al. Tristetraprolin controls the stability of cIAP2 mRNA through binding to the 3'UTR of cIAP2 mRNA. Biochem Biophys Res Commun 400:46-52 (2010).
Read more (PubMed: 20691152) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"