Overview

  • Product nameAnti-TIS11D antibody
    See all TIS11D primary antibodies
  • Description
    Rabbit polyclonal to TIS11D
  • Specificityab70775 detects endogenous levels of total TIS11D protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human TIS11D

  • Positive control
    • A549 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab70775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 52 kDa).
ELISA 1/20000.

Target

  • FunctionmRNA-binding protein that plays a key role in self-renewal of erythroid cells in response to glucocorticoids. Specifically binds to the AU-rich element (ARE) in the 3'-UTR of target mRNAs, promoting their deadenylation and degradation (PubMed:14981510). Specifically expressed in burst-forming unit-erythroid (BFU-E) progenitors in response to glucocorticoids and acts as a negative regulator of erythroid cell differentiation: promotes self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation. Down-regulated during erythroid differentiation from the BFU-E stage, stabilizing mRNAs required for terminal differentiation.
  • Involvement in diseaseDefects in ZFP36L2 may be a cause of leukemias. Frameshfits mutations disrupting ZFP36L2 have been found in a patient with acute myeloid leukemia (PubMed:21109922).
  • Sequence similaritiesContains 2 C3H1-type zinc fingers.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRF2 antibody
    • Butyrate response factor 2 antibody
    • C3H1 type-like 2 antibody
    • EGF response factor 2 antibody
    • EGF-response factor 2 antibody
    • ERF 2 antibody
    • ERF-2 antibody
    • ERF2 antibody
    • Protein TIS11D antibody
    • RNF162C antibody
    • TIS11D antibody
    • TISD_HUMAN antibody
    • ZFP36 like 2 antibody
    • ZFP36 ring finger protein like 2 antibody
    • ZFP36-like 2 antibody
    • Zfp36l2 antibody
    • Zinc finger protein 36 antibody
    • Zinc finger protein 36, C3H type like 1 antibody
    • zinc finger protein 36, C3H type like 2 antibody
    • Zinc finger protein 36, C3H1 type like 2 antibody
    • Zinc finger protein, C3H type, 36 like 2 antibody
    see all

Anti-TIS11D antibody images

  • All lanes : Anti-TIS11D antibody (ab70775) at 1/500 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-TIS11D antibody (ab70775)

This product has been referenced in:
  • Zhang L  et al. ZFP36L2 is required for self-renewal of early burst-forming unit erythroid progenitors. Nature 499:92-6 (2013). WB, IP ; Mouse . Read more (PubMed: 23748442) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

I have heard back from the lab regarding ab83579:


Crossreactivity with TISIIB and TISIID isn't something we have tested in house but an immunogen search shows that both TISIIB and TISIID only have 4 resi...

Read More

Thank you for contacting us.

I have received the following information regading 2 of the antibodies. For ab83579, I am still waiting for more information.

ab70775:
The antibody was raised against human TIS11D. This antibody has be...

Read More

Thank you for contacting us.





The immunogen sequence used to create this antibody is from aa231-270 ofhuman TIS11D. This sequence contains a 15aa span which has a 70% homology with TIS11B. At this level, we do not cons...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"